MRI of prostate stem cell antigen expression in prostate tumors

Jing Ren, Zhuoli Zhang, Fang Wang, Yong Yang, Ying Liu, Guangquan Wei, Angang Yang, Rui Zhang, Yi Huan*, Yali Cui, Andrew C. Larson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Background: The prostate stem cell antigen (PSCA) is broadly overexpressed on the surface of prostate cancer cells. Materials & methods: Anti-human PSCA monoclonal antibody (mAb 7F5) was bound to Fe3O4/Au (GoldMag) nanoparticles to serve as a PSCA-specific molecular MRI probe (mAb 7F5@GoldMag) for in vivo detection of prostate cancer cells. First, the efficacy of the antibody immobilization for the binding was assessed. Next, PC-3 (human prostate cancer cell line with PSCA overexpression) tumor-bearing mice were injected with mAb 7F5@GoldMag for MRI measurements while using mouse anti-human IgG bound to the particles (IgG@GoldMag) to serve as a nonspecific control. MRI examinations were conducted before and after injection of these probes at 6, 12 and 24 h; T2-weighted signal intensity within the tumors was measured. Results: Targeted binding of the mAb 7F5@GoldMag probe to PC-3 tumors was verified with optical images and MRI; selective binding was not observed for the nonspecific IgG@GoldMag probe. Conclusion: MRI measurements suggest the promising efficacy of this new approach for targeted molecular imaging of prostate tumors. Original submitted 5 March 2011; Revised submitted 4 July 201.

Original languageEnglish (US)
Pages (from-to)691-703
Number of pages13
JournalNanomedicine
Volume7
Issue number5
DOIs
StatePublished - May 1 2012

Keywords

  • imaging probe
  • magnetic resonance imaging
  • prostate cancer diagnosis
  • prostate stem cell antigen

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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