MS-based ligand binding assays with speed, sensitivity, and specificity

Michael J. Roth, Jaekuk Kim, Erica M. Maresh, Daniel A. Plymire, John R. Corbett, Junmei Zhang, Steven M. Patrie*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Immunoassays are widely used in biochemical/clinical laboratories owing to their simplicity, speed, and sensitivity. We combined self-assembled monolayer-based immunoassays with MALDI-TOF MS to show that high-fidelity surface preparations with a novel matrix deposition/crystallization technique permits quantitative analysis of monolayer-bound antigens at picomolar detection limits. Calibration curves for intact proteins are possible over a broad concentration range and improved specificity of MS-immunoassays is highlighted by simultaneous label-free quantitation of ligand-bound protein complexes.

Original languageEnglish (US)
Pages (from-to)3143-3146
Number of pages4
JournalProteomics
Volume12
Issue number21
DOIs
StatePublished - Nov 2012

Keywords

  • Immunodetection
  • MS
  • Protein chips
  • Protein profiling
  • Quantitative analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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