Msx2 and p21CIP1/WAF1 mediate the proapoptotic effects of bone morphogenetic protein-4 on ventricular zone progenitor cells

Nipan Israsena, John A. Kessler*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Treatment of cultured ventricular zone (VZ) progenitor cells with bone morphogenetic protein-4 (BMP4) promoted cell death in a dose-dependent manner. VZ progenitor cells became progressively more resistant to the proapoptotic effects of BMP4 between E10 and E16, and, by E18 and thereafter, BMP4 treatment no longer led to progenitor cell death. BMP4 treatment of E13 progenitor cells promoted expression of msx2 and p21CIP1/WAF1 (p21) and inhibition of expression of either gene prevented BMP4-mediated apoptosis. Treatment of E18 cells with BMP4 failed to induce apoptosis but still induced expression of low levels of msx2 and p21. Knockout of bax significantly reduced but did not prevent BMP4-mediated death of E13 murine progenitor cells. These observations indicate that msx2 and p21 mediate the proapoptotic effects of BMP4 on VZ progenitor cells and that each gene is necessary but insufficient to promote apoptosis.

Original languageEnglish (US)
Pages (from-to)803-809
Number of pages7
JournalJournal of Neuroscience Research
Volume69
Issue number6
DOIs
StatePublished - Sep 15 2002

Keywords

  • Apoptosis
  • BMP4
  • Msx2
  • P2
  • Progenitor cell

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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