Multifunctional 3D-Printed Wound Dressings

Moien Alizadehgiashi; Carine R. Nemr; Mahshid Chekini; Daniel Pinto Ramos; Nitesh Mittal; Sharif U. Ahmed; Nancy Khuu; Shana O. Kelley; Eugenia Kumacheva

doi:10.1021/acsnano.1c04499

Multifunctional 3D-Printed Wound Dressings

Moien Alizadehgiashi, Carine R. Nemr, Mahshid Chekini, Daniel Pinto Ramos, Nitesh Mittal, Sharif U. Ahmed, Nancy Khuu, Shana O. Kelley, Eugenia Kumacheva^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

132 Scopus citations

Abstract

Personalized wound dressings provide enhanced healing for different wound types; however multicomponent wound dressings with discretely controllable delivery of different biologically active agents are yet to be developed. Here we report 3D-printed multicomponent biocomposite hydrogel wound dressings that have been selectively loaded with small molecules, metal nanoparticles, and proteins for independently controlled release at the wound site. Hydrogel wound dressings carrying antibacterial silver nanoparticles and vascular endothelial growth factor with predetermined release profiles were utilized to study the physiological response of the wound in a mouse model. Compared to controls, the application of dressings resulted in improvement in granulation tissue formation and differential levels of vascular density, dependent on the release profile of the growth factor. Our study demonstrates the versatility of the 3D-printed hydrogel dressings that can yield varied physiological responses in vivo and can further be adapted for personalized treatment of various wound types.

Original language	English (US)
Pages (from-to)	12375-12387
Number of pages	13
Journal	ACS nano
Volume	15
Issue number	7
DOIs	https://doi.org/10.1021/acsnano.1c04499
State	Published - Jul 27 2021

Funding

This work was supported by NSERC Discovery grants (S.O.K. and E.K.), an NSERC Strategic grant (E.K.), and a CIHR Foundation grant (S.O.K.). M.A. thanks NSERC for the Vanier Canada Graduate Scholarship. C.R.N. and N.K. thank NSERC for the Alexander Graham Bell Canada Graduate Scholarship (CGS-D). This work was supported by the Society for Laboratory Automation and Screening, under award number SLASFG2019. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect those of the Society for Laboratory Automation and Screening. E.K. is grateful to the Canada Research Chair program (NSERC Canada). The authors thank I. Gourevich (Centre for Nanostructure Imaging) for assistance in electron microscopy imaging, D. Bogojevic from Saint Michael’s Hospital for 3D printing of the microfluidics devices, A. Al-Mahrouki (Centre for Pharmaceutical Oncology) for assistance with Cytation 5 multimode reader measurements, and M. Larsen from Mbed Pathology for histological assessment. The authors thank E. Tumarkin, M. A. Winnik, R. Cairns, E. Slavko, N. Obhi, and S. Gomis for fruitful discussions.

Keywords

cellulose nanocrystals
drug delivery
extrusion-based 3D printing
nanocolloidal hydrogel
personalized wound care
wound dressing

ASJC Scopus subject areas

General Materials Science
General Engineering
General Physics and Astronomy

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10.1021/acsnano.1c04499

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title = "Multifunctional 3D-Printed Wound Dressings",

abstract = "Personalized wound dressings provide enhanced healing for different wound types; however multicomponent wound dressings with discretely controllable delivery of different biologically active agents are yet to be developed. Here we report 3D-printed multicomponent biocomposite hydrogel wound dressings that have been selectively loaded with small molecules, metal nanoparticles, and proteins for independently controlled release at the wound site. Hydrogel wound dressings carrying antibacterial silver nanoparticles and vascular endothelial growth factor with predetermined release profiles were utilized to study the physiological response of the wound in a mouse model. Compared to controls, the application of dressings resulted in improvement in granulation tissue formation and differential levels of vascular density, dependent on the release profile of the growth factor. Our study demonstrates the versatility of the 3D-printed hydrogel dressings that can yield varied physiological responses in vivo and can further be adapted for personalized treatment of various wound types.",

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author = "Moien Alizadehgiashi and Nemr, {Carine R.} and Mahshid Chekini and {Pinto Ramos}, Daniel and Nitesh Mittal and Ahmed, {Sharif U.} and Nancy Khuu and Kelley, {Shana O.} and Eugenia Kumacheva",

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AU - Nemr, Carine R.

AU - Chekini, Mahshid

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AU - Mittal, Nitesh

AU - Ahmed, Sharif U.

AU - Khuu, Nancy

AU - Kelley, Shana O.

AU - Kumacheva, Eugenia

N1 - Funding Information: This work was supported by NSERC Discovery grants (S.O.K. and E.K.), an NSERC Strategic grant (E.K.), and a CIHR Foundation grant (S.O.K.). M.A. thanks NSERC for the Vanier Canada Graduate Scholarship. C.R.N. and N.K. thank NSERC for the Alexander Graham Bell Canada Graduate Scholarship (CGS-D). This work was supported by the Society for Laboratory Automation and Screening, under award number SLASFG2019. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect those of the Society for Laboratory Automation and Screening. E.K. is grateful to the Canada Research Chair program (NSERC Canada). The authors thank I. Gourevich (Centre for Nanostructure Imaging) for assistance in electron microscopy imaging, D. Bogojevic from Saint Michael’s Hospital for 3D printing of the microfluidics devices, A. Al-Mahrouki (Centre for Pharmaceutical Oncology) for assistance with Cytation 5 multimode reader measurements, and M. Larsen from Mbed Pathology for histological assessment. The authors thank E. Tumarkin, M. A. Winnik, R. Cairns, E. Slavko, N. Obhi, and S. Gomis for fruitful discussions. Publisher Copyright: ©

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N2 - Personalized wound dressings provide enhanced healing for different wound types; however multicomponent wound dressings with discretely controllable delivery of different biologically active agents are yet to be developed. Here we report 3D-printed multicomponent biocomposite hydrogel wound dressings that have been selectively loaded with small molecules, metal nanoparticles, and proteins for independently controlled release at the wound site. Hydrogel wound dressings carrying antibacterial silver nanoparticles and vascular endothelial growth factor with predetermined release profiles were utilized to study the physiological response of the wound in a mouse model. Compared to controls, the application of dressings resulted in improvement in granulation tissue formation and differential levels of vascular density, dependent on the release profile of the growth factor. Our study demonstrates the versatility of the 3D-printed hydrogel dressings that can yield varied physiological responses in vivo and can further be adapted for personalized treatment of various wound types.

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ER -

Multifunctional 3D-Printed Wound Dressings

Abstract

Funding

Keywords

ASJC Scopus subject areas

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