TY - JOUR
T1 - Multilineage differentiation activity by cells isolated from umbilical cord blood
T2 - Expression of bone, fat, and neural markers
AU - Goodwin, H. S.
AU - Bicknese, A. R.
AU - Chien, S. N.
AU - Bogucki, B. D.
AU - Oliver, D. A.
AU - Quinn, C. O.
AU - Wall, D. A.
N1 - Funding Information:
This work was supported by the Fleur-de-Lis Foundation, Cardinal Glennon Children’s Hospital Foundation. The authors thank the St. Louis Cord Blood Bank and the many phy sicians, nurses, and parents who participate in the program. We thank Alastair Mackay and colleagues at Osiris Therapeutics for sharing MSCs and ideas; V. Rocha at Hopital Saint-Louis, Paris, for his insights into endothelial cell precursors; and R. J. Fallon at Riley Hospital for Children for his review of the manuscript.
PY - 2001
Y1 - 2001
N2 - The stromal cell population in bone marrow has been the focus of much attention since it has been shown that this cell population can be expanded and differentiated into cells with the phenotype of bone, cartilage, muscle, stroma, neural, and fat cells. We evaluated umbilical cord blood (UCB) for the presence of these cells. From the mononuclear fraction of UCB, we demonstrated the presence of a subset of cells that have been maintained in continuous culture for more than 6 months (>10 passages). These adherent cell populations express adhesion molecules CD13+, CD29+, and CD44+, but not antigens of hematopoietic differentiation. Exposure of these cells to osteogenic agents resulted in an increase in expression of alkaline phosphatase and the appearance of hydroxyapatite nodules by Von Kossa staining. Incubation with adipogenic agents resulted in morphological change and staining with Oil Red O. In addition, when exposed to basic fibroblast growth factor and human epidermal growth factor the cells underwent changes consistent with cells of neural origin. These changes were demonstrated by a combination of immunofluorescent labeling and Western immunoblots for neural-specific markers. Thus, similar to what has been previously reported with bone marrow, cord blood contains a population of cells that can be expanded in culture and are able to express the phenotype of multiple lineages. Cord blood multilineage cells are slower to establish in culture, have a lower precursor frequency and a lower level of bone antigen expression, and lack constitutive expression of neural antigens when compared to bone marrow, suggesting a more primitive population. Cord blood may prove to be a new source of cells for cellular therapeutics for stromal, bone, and, potentially, neural repair.
AB - The stromal cell population in bone marrow has been the focus of much attention since it has been shown that this cell population can be expanded and differentiated into cells with the phenotype of bone, cartilage, muscle, stroma, neural, and fat cells. We evaluated umbilical cord blood (UCB) for the presence of these cells. From the mononuclear fraction of UCB, we demonstrated the presence of a subset of cells that have been maintained in continuous culture for more than 6 months (>10 passages). These adherent cell populations express adhesion molecules CD13+, CD29+, and CD44+, but not antigens of hematopoietic differentiation. Exposure of these cells to osteogenic agents resulted in an increase in expression of alkaline phosphatase and the appearance of hydroxyapatite nodules by Von Kossa staining. Incubation with adipogenic agents resulted in morphological change and staining with Oil Red O. In addition, when exposed to basic fibroblast growth factor and human epidermal growth factor the cells underwent changes consistent with cells of neural origin. These changes were demonstrated by a combination of immunofluorescent labeling and Western immunoblots for neural-specific markers. Thus, similar to what has been previously reported with bone marrow, cord blood contains a population of cells that can be expanded in culture and are able to express the phenotype of multiple lineages. Cord blood multilineage cells are slower to establish in culture, have a lower precursor frequency and a lower level of bone antigen expression, and lack constitutive expression of neural antigens when compared to bone marrow, suggesting a more primitive population. Cord blood may prove to be a new source of cells for cellular therapeutics for stromal, bone, and, potentially, neural repair.
KW - Differentiation
KW - Multilineage cells
KW - Nonhematopoietic progenitors
KW - Umbilical cord blood
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U2 - 10.1053/bbmt.2001.v7.pm11760145
DO - 10.1053/bbmt.2001.v7.pm11760145
M3 - Article
C2 - 11760145
AN - SCOPUS:0035199276
SN - 1083-8791
VL - 7
SP - 581
EP - 588
JO - Biology of Blood and Marrow Transplantation
JF - Biology of Blood and Marrow Transplantation
IS - 11
ER -