Multimodal Magnetic Nanoclusters for Gene Delivery, Directed Migration, and Tracking of Stem Cells

Ji Sun Park, Wooram Park, Sin Jung Park, Andrew C. Larson, Dong Hyun Kim, Keun Hong Park*

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

This study develops multimodal magnetic nanoclusters (M-MNCs) for gene transfer, directed migration, and tracking of human mesenchymal stem cells (hMSCs). The M-MNCs are designed with 5 nm iron oxide nanoparticles and a fluorescent dye (i.e., Rhodamine B) in the matrix of the Food and Drug Administration approved polymer poly(lactide-co-glycolide) using a nanoemulsion method. The synthesized M-MNCs have a hydrodynamic diameter of ≈150 nm, are internalized by stem cells via endocytosis, and deliver genes with high efficiency. The cellular internalization and gene expression efficiency of the clustered nanoparticles are significantly higher than that of single nanoparticles. The M-MNC-labeled hMSCs migrate upon application of a magnetic force and can be visualized by both optical and magnetic resonance (MR) imaging. In animal models, the M-MNC-labeled hMSCs are also successfully tracked using optical and MR imaging. Thus, the M-MNCs not only allow the efficient delivery of genes to stem cells but also the tracking of cells in animal models. Taken together, the results show that this new type of nanocomposite can be of great help in future stem cell research and in the development of cell-based therapeutic agents.

Original languageEnglish (US)
Article number1700396
JournalAdvanced Functional Materials
Volume27
Issue number25
DOIs
StatePublished - Jul 5 2017

Keywords

  • gene delivery
  • magnetic cell migration
  • magnetic nanoparticles
  • stem cell tracking
  • tissue engineering

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

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