Multimodal photoacoustic ophthalmoscopy in mouse

Wei Song, Qing Wei, Liang Feng, Vijay Sarthy, Shuliang Jiao, Xiaorong Liu, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Photoacoustic ophthalmoscopy (PAOM) is a novel imaging technology that measures optical absorption in the retina. The capability of PAOM can be further enhanced if it could image mouse eyes, because mouse models are widely used for various retinal diseases. The challenges in achieving high-quality imaging of mouse retina, however, come from the much smaller eyeball size. Here, we report an optimized imaging system, which integrates PAOM, spectral-domain optical coherence tomography (SD-OCT), and autofluorescence-scanning laser ophthalmoscopy (AF-SLO), for mouse eyes. Its multimodal capability was demonstrated by imaging transgenic Nrl-GFP mice that express green fluorescent protein (GFP) in photoreceptors. SD-OCT provided guidance of optical alignment for PAOM and AF-SLO, and complementary contrast with high depth-resolution retinal cross sections. PAOM visualized the retinal vasculature and retinal pigment epithelium melanin, and AF-SLO measured GFP-expressing in retinal photoreceptors. The in vivo imaging results were verified by histology and confocal microscopy.

Original languageEnglish (US)
Pages (from-to)505-512
Number of pages8
JournalJournal of Biophotonics
Issue number6-7
StatePublished - Jun 2013


  • Optical coherence tomography
  • Photoacoustic ophthalmoscopy
  • Retinal imaging
  • Scanning laser ophthalmoscopy

ASJC Scopus subject areas

  • General Chemistry
  • General Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Materials Science
  • General Physics and Astronomy


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