Multiphoton imaging approaches for studying striatal dendritic excitability

Joshua L. Plotkin, D. James Surmeier

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

As the main input nucleus to the basal ganglia, the striatum is responsible for receiving and integrating highly convergent afferents to ultimately guide action selection and movement initiation. Although the majority of this synaptic integration occurs in the dendrites of striatal projection neurons (SPNs), their thin diameter makes them inaccessible with traditional recording electrodes. Recent advances in optical imaging technologies have allowed us and others to start lifting the veil on the mechanisms governing synaptic integration in the striatum by enabling direct dendritic measurements and manipulations. Here we describe how our lab has approached combining 2-photon imaging and photolysis with electrophysiological recordings to study dendritic excitability and synaptic integration in the striatum.

Original languageEnglish (US)
Title of host publicationPatch-Clamp Methods and Protocols
PublisherHumana Press Inc.
Pages171-182
Number of pages12
ISBN (Print)9781493910953
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1183
ISSN (Print)1064-3745

Keywords

  • 2-Photon imaging
  • 2-Photon uncaging
  • Calcium imaging
  • Dendritic morphology
  • Striatum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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