Multiphoton microscopy: A retrospective focused on the visualization of embryo division dynamics

D. L. Wokosin*

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations

Abstract

The development of laser scanning fluorescence microscopy will be outlined. The focus will be technical instrumentation applied to solve biological problems through dynamic, high-resolution imaging. Laser scanning confocal microscopy will be presented first, followed by two-photon excitation fluorescence microscopy. Ideal imaging modes for two-photon imaging will be highlighted: deep tissue imaging and live cell imaging. Contributions from selected pioneers over the last decade of multi-photon imaging field will be highlighted in specific biological applications areas where two-photon imaging has already been established as the best (or only) option: intact tissues, developing embryos, and whole animal studies. The specific, unifying thread will focus on the quest for the observation of microtubule dynamics during the first few, asymmetric cell divisions in Caenorhabditis elegans embryos.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4262
DOIs
StatePublished - Jan 1 2001
EventMultiphoton Microscopy in the Biomedical Sciences - San Jose, CA, United States
Duration: Jan 21 2001Jan 23 2001

Keywords

  • Fluorescence microscopy
  • Laser scanning confocal imaging
  • Multiphoton excitation
  • Ultra-fast laser applications

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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