Multiple enhancer domains in the 3′ terminus of the prague strain of rous sarcoma virus

Laimonis A. Laimins*, Philip Tsichlis, George Khoury

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

The 3′ terminal region of the Prague strain of Rous sarcoma virus (PrRSV) contains at least three distinct domains that comprise two func tional enhancer elements. Two of these domains (designated B and C) are found in the U3 region of the 3′ long terminal repeat (LTR) while the third (designated A) is located in the sequences immediately preceding the LTR termed XSR sequences. Combinations of adjacent domains [e.g., (A + B or B + C)] are capable of activating the expression of the SV4O early promoter (21 bp repeats and TATA box) coupled to coding sequences from the prokaryotic gene chloramphenicol acetyltransferase (CAT) while a single domain is inactive. Furthermore, duplication or triplication of the central domain B restores activity. The related, Schmidt-Ruppin, strain of RSV, contains an almost identical 3′ LTR element, but differs in the enhan cer sequences immediately preceding the 3′ LTR. A model is presented in which the sequence differences may contribute to the difference in disease spectrum of transformation defective (td) variants of these viruses.

Original languageEnglish (US)
Pages (from-to)6427-6442
Number of pages16
JournalNucleic acids research
Volume12
Issue number16
DOIs
StatePublished - Aug 24 1984

ASJC Scopus subject areas

  • Genetics

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