Multiple microscopy modalities applied to a sea urchin tooth fragment

S. R. Stock*, K. Ignatiev, T. Dahl, J. Barss, K. Fezzaa, A. Veis, W. K. Lee, F. De Carlo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Two synchrotron X-ray microscopy methods, phase-contrast micro-radiography (the propagation method) and absorption microCT (high-resolution computed tomography or microtomography), and laser-scanning confocal microscopy (visible wavelength) were used to study a fragment of the keel of a tooth of the sea urchin Lytechinus variegatus. Stripes observed in the phase-contrast images of the fragment were also seen in confocal micrographs. MicroCT showed that the stripes were due to two parallel planar arrays of low-absorption channels within the bulk of the keel. In the phase microradiographs, maximum contrast stripes appear when a channel image from one row coincides with a channel image from the second row; otherwise, contrast is minimal. Long channels do not appear to have been observed previously in keels of sea urchin teeth.

Original languageEnglish (US)
Pages (from-to)393-397
Number of pages5
JournalJournal of Synchrotron Radiation
Volume10
Issue number5
DOIs
StatePublished - Sep 1 2003

Keywords

  • Calcite
  • Computed tomography
  • Keel
  • Sea urchins
  • Teeth
  • X-ray microCT
  • X-ray phase contrast

ASJC Scopus subject areas

  • Radiation
  • Nuclear and High Energy Physics
  • Instrumentation

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