Multiple potential intragenic regulatory elements in the CFTR gene

David J. Smith; Hugh N. Nuthall; Margaret E. Majetti; Ann Harris

doi:10.1006/geno.1999.6086

Multiple potential intragenic regulatory elements in the CFTR gene

David J. Smith, Hugh N. Nuthall, Margaret E. Majetti, Ann Harris

Pediatrics

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The CFTR gene exhibits a complex pattern of expression that shows temporal and spatial regulation though the control mechanisms have not been fully elucidated. We have mapped DNase I hypersensitive sites (DHS) flanking the CFTR gene to identify potential regulatory elements. We previously characterized DHS at -79.5 and -20.9 kb with respect to the CFTR translational start site, DHS 3' to the gene at 4574 + 5.4-7.4 and 4574 + 15.6 kb, and a regulatory element in the first intron of the gene at 185 + 10 kb. We generated a cosmid contig to provide probes to evaluate the whole of the CFTR gene for DHS and have now mapped novel sites in introns 2, 3, 10, 16, 17a, 18, 20, and 21. These DHS show different patterns of cell-specific expression.

Original language	English (US)
Pages (from-to)	90-96
Number of pages	7
Journal	Genomics
Volume	64
Issue number	1
DOIs	https://doi.org/10.1006/geno.1999.6086
State	Published - Feb 15 2000

Funding

This work was supported by the Cystic Fibrosis Trust, Medical Research Council, Wellcome Trust, and AFLM. H.N. was a CFT research student. We are grateful to the Reference Library Database, Max-Planck-Institut fur Molekulare Genetik, Berlin, for access to reference library filters, to J. Rommens for the cJ21 and cW44 cosmids, and to S. Shackleton and R. Rowntree for assistance.

ASJC Scopus subject areas

Genetics

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10.1006/geno.1999.6086

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title = "Multiple potential intragenic regulatory elements in the CFTR gene",

abstract = "The CFTR gene exhibits a complex pattern of expression that shows temporal and spatial regulation though the control mechanisms have not been fully elucidated. We have mapped DNase I hypersensitive sites (DHS) flanking the CFTR gene to identify potential regulatory elements. We previously characterized DHS at -79.5 and -20.9 kb with respect to the CFTR translational start site, DHS 3' to the gene at 4574 + 5.4-7.4 and 4574 + 15.6 kb, and a regulatory element in the first intron of the gene at 185 + 10 kb. We generated a cosmid contig to provide probes to evaluate the whole of the CFTR gene for DHS and have now mapped novel sites in introns 2, 3, 10, 16, 17a, 18, 20, and 21. These DHS show different patterns of cell-specific expression.",

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Multiple potential intragenic regulatory elements in the CFTR gene

Abstract

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ASJC Scopus subject areas

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