Multiple thiol-anchor capped DNA-gold nanoparticle conjugates

Zhi Li; Rongchao Jin; Chad A. Mirkin; Robert L. Letsinger

doi:10.1093/nar/30.7.1558

Multiple thiol-anchor capped DNA-gold nanoparticle conjugates

Zhi Li, Rongchao Jin, Chad A. Mirkin^*, Robert L. Letsinger

^*Corresponding author for this work

Robert H. Lurie Comprehensive Cancer Center

Research output: Contribution to journal › Review article › peer-review

346 Scopus citations

Abstract

We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.

Original language	English (US)
Pages (from-to)	1558-1562
Number of pages	5
Journal	Nucleic acids research
Volume	30
Issue number	7
DOIs	https://doi.org/10.1093/nar/30.7.1558
State	Published - Apr 1 2002

Funding

The authors acknowledge Mr Gang Lu and Ms Min Zhao for help in synthesizing oligonucleotides. C.A.M. acknowledges the AFOSR, ARO, DARPA and NSF for support of this research. R.L.L. acknowledges NIH (grant GM 57356) for the support of this research.

ASJC Scopus subject areas

Genetics

Access to Document

10.1093/nar/30.7.1558

Cite this

@article{7a225621c0554a1396a4d91d51172a3b,

title = "Multiple thiol-anchor capped DNA-gold nanoparticle conjugates",

abstract = "We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.",

author = "Zhi Li and Rongchao Jin and Mirkin, {Chad A.} and Letsinger, {Robert L.}",

note = "Funding Information: The authors acknowledge Mr Gang Lu and Ms Min Zhao for help in synthesizing oligonucleotides. C.A.M. acknowledges the AFOSR, ARO, DARPA and NSF for support of this research. R.L.L. acknowledges NIH (grant GM 57356) for the support of this research.",

year = "2002",

month = apr,

day = "1",

doi = "10.1093/nar/30.7.1558",

language = "English (US)",

volume = "30",

pages = "1558--1562",

journal = "Nucleic acids research",

issn = "0305-1048",

publisher = "Oxford University Press",

number = "7",

}

TY - JOUR

T1 - Multiple thiol-anchor capped DNA-gold nanoparticle conjugates

AU - Li, Zhi

AU - Jin, Rongchao

AU - Mirkin, Chad A.

AU - Letsinger, Robert L.

N1 - Funding Information: The authors acknowledge Mr Gang Lu and Ms Min Zhao for help in synthesizing oligonucleotides. C.A.M. acknowledges the AFOSR, ARO, DARPA and NSF for support of this research. R.L.L. acknowledges NIH (grant GM 57356) for the support of this research.

PY - 2002/4/1

Y1 - 2002/4/1

N2 - We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.

AB - We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.

UR - http://www.scopus.com/inward/record.url?scp=0036529538&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036529538&partnerID=8YFLogxK

U2 - 10.1093/nar/30.7.1558

DO - 10.1093/nar/30.7.1558

M3 - Review article

C2 - 11917016

AN - SCOPUS:0036529538

SN - 0305-1048

VL - 30

SP - 1558

EP - 1562

JO - Nucleic acids research

JF - Nucleic acids research

IS - 7

ER -

Multiple thiol-anchor capped DNA-gold nanoparticle conjugates

Abstract

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this