Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes

Jared O. Kafader; Rafael D. Melani; Kenneth R. Durbin; Bon Ikwuagwu; Bryan P. Early; Ryan T. Fellers; Steven C. Beu; Vlad Zabrouskov; Alexander A. Makarov; Joshua T. Maze; Deven L. Shinholt; Ping F. Yip; Danielle Tullman-Ercek; Michael W. Senko; Philip D. Compton; Neil L. Kelleher

doi:10.1038/s41592-020-0764-5

Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes

Jared O. Kafader, Rafael D. Melani, Kenneth R. Durbin, Bon Ikwuagwu, Bryan P. Early, Ryan T. Fellers, Steven C. Beu, Vlad Zabrouskov, Alexander A. Makarov, Joshua T. Maze, Deven L. Shinholt, Ping F. Yip, Danielle Tullman-Ercek, Michael W. Senko, Philip D. Compton^*, Neil L. Kelleher

^*Corresponding author for this work

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I²MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.

Original language	English (US)
Pages (from-to)	391-394
Number of pages	4
Journal	Nature Methods
Volume	17
Issue number	4
DOIs	https://doi.org/10.1038/s41592-020-0764-5
State	Published - Apr 1 2020

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Kafader, J. O., Melani, R. D., Durbin, K. R., Ikwuagwu, B., Early, B. P., Fellers, R. T., Beu, S. C., Zabrouskov, V., Makarov, A. A., Maze, J. T., Shinholt, D. L., Yip, P. F., Tullman-Ercek, D., Senko, M. W., Compton, P. D., & Kelleher, N. L. (2020). Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes. Nature Methods, 17(4), 391-394. https://doi.org/10.1038/s41592-020-0764-5

Kafader, Jared O. ; Melani, Rafael D. ; Durbin, Kenneth R. ; Ikwuagwu, Bon ; Early, Bryan P. ; Fellers, Ryan T. ; Beu, Steven C. ; Zabrouskov, Vlad ; Makarov, Alexander A. ; Maze, Joshua T. ; Shinholt, Deven L. ; Yip, Ping F. ; Tullman-Ercek, Danielle ; Senko, Michael W. ; Compton, Philip D. ; Kelleher, Neil L. / Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes. In: Nature Methods. 2020 ; Vol. 17, No. 4. pp. 391-394.

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abstract = "An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I2MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.",

author = "Kafader, {Jared O.} and Melani, {Rafael D.} and Durbin, {Kenneth R.} and Bon Ikwuagwu and Early, {Bryan P.} and Fellers, {Ryan T.} and Beu, {Steven C.} and Vlad Zabrouskov and Makarov, {Alexander A.} and Maze, {Joshua T.} and Shinholt, {Deven L.} and Yip, {Ping F.} and Danielle Tullman-Ercek and Senko, {Michael W.} and Compton, {Philip D.} and Kelleher, {Neil L.}",

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doi = "10.1038/s41592-020-0764-5",

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Kafader, JO, Melani, RD, Durbin, KR, Ikwuagwu, B, Early, BP, Fellers, RT, Beu, SC, Zabrouskov, V, Makarov, AA, Maze, JT, Shinholt, DL, Yip, PF, Tullman-Ercek, D, Senko, MW, Compton, PD & Kelleher, NL 2020, 'Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes', Nature Methods, vol. 17, no. 4, pp. 391-394. https://doi.org/10.1038/s41592-020-0764-5

Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes. / Kafader, Jared O.; Melani, Rafael D.; Durbin, Kenneth R.; Ikwuagwu, Bon; Early, Bryan P.; Fellers, Ryan T.; Beu, Steven C.; Zabrouskov, Vlad; Makarov, Alexander A.; Maze, Joshua T.; Shinholt, Deven L.; Yip, Ping F.; Tullman-Ercek, Danielle; Senko, Michael W.; Compton, Philip D.; Kelleher, Neil L.

In: Nature Methods, Vol. 17, No. 4, 01.04.2020, p. 391-394.

Research output: Contribution to journal › Article

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AU - Maze, Joshua T.

AU - Shinholt, Deven L.

AU - Yip, Ping F.

AU - Tullman-Ercek, Danielle

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AU - Compton, Philip D.

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