@article{3196e5bf4be44effaa5e0c99badf4329,
title = "Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes",
abstract = "An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I2MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.",
author = "Kafader, {Jared O.} and Melani, {Rafael D.} and Durbin, {Kenneth R.} and Bon Ikwuagwu and Early, {Bryan P.} and Fellers, {Ryan T.} and Beu, {Steven C.} and Vlad Zabrouskov and Makarov, {Alexander A.} and Maze, {Joshua T.} and Shinholt, {Deven L.} and Yip, {Ping F.} and Danielle Tullman-Ercek and Senko, {Michael W.} and Compton, {Philip D.} and Kelleher, {Neil L.}",
note = "Funding Information: This work was funded by the Intensifying Innovation program from Thermo Fisher Scientific and was carried out in collaboration with the National Resource for Translational and Developmental Proteomics under Grant P41 GM108569 from the National Institute of General Medical Sciences, National Institutes of Health with additional support from the Sherman Fairchild Foundation, and the instrumentation award (S10OD025194) from NIH Office of Director. In addition, we would like to thank Luca Fornelli and Timothy K. Toby for collecting and analyzing the HEK-293 LC–MS runs utilized for our intact-mass-tag I2MS analysis.",
year = "2020",
month = apr,
day = "1",
doi = "10.1038/s41592-020-0764-5",
language = "English (US)",
volume = "17",
pages = "391--394",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "4",
}