Multiplexed nanoflares: MRNA detection in live cells

Andrew E. Prigodich, Pratik S. Randeria, William E. Briley, Nathaniel J. Kim, Weston L. Daniel, David A. Giljohann, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

222 Scopus citations

Abstract

We report the development of the multiplexed nanoflare, a nanoparticle agent that is capable of simultaneously detecting two distinct mRNA targets inside a living cell. These probes are spherical nucleic acid (SNA) gold nanoparticle (Au NP) conjugates consisting of densely packed and highly oriented oligonucleotide sequences, many of which are hybridized to a reporter with a distinct fluorophore label and each complementary to its corresponding mRNA target. When multiplexed nanoflares are exposed to their targets, they provide a sequence specific signal in both extra- and intracellular environments. Importantly, one of the targets can be used as an internal control, improving detection by accounting for cellto- cell variations in nanoparticle uptake and background. Compared to single-component nanoflares, these structures allow one to determine more precisely relative mRNA levels in individual cells, improving cell sorting and quantification.

Original languageEnglish (US)
Pages (from-to)2062-2066
Number of pages5
JournalAnalytical Chemistry
Volume84
Issue number4
DOIs
StatePublished - Feb 21 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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