Multispectral imaging approach to the diagnosis of a CD20+ cutaneous T-cell lymphoproliferative disorder: A case report

Katrin A. Salva*, Daniel Bennett, Jack Longley, Joan Guitart, Gary S. Wood

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Expression of the pan B-cell marker CD20 by T-cell lymphoproliferative disorders is exceedingly rare. We present a 52-year-old man with a unilesional cutaneous CD20+ T-cell lymphoproliferative disorder. Multispectral imaging analysis of CD3-CD20 double-stained lesional tissue sections allowed (1) the visualization of double-positive T lymphocytes in situ with sensitivity superior to that of conventional immunohistochemistry and (2) the quantitative assessment of marker coexpression. Here, 23% of CD3+ signals in the patient's lesion were also CD20+, whereas 38% of CD20 + signals were also CD3+. In contrast, both parameters were below 1% in the tonsil control. Overall, the percentage of doublepositive cells in lesional skin was 35%, although only 0.4% of such cells were detected in the tonsil. This is the first demonstration of aberrant CD20 expression by skin-infiltrating T cells using multispectral imaging.

Original languageEnglish (US)
Pages (from-to)e116-e121
JournalAmerican Journal of Dermatopathology
Volume37
Issue number10
DOIs
StatePublished - Oct 1 2015

Keywords

  • CD20
  • Cutaneous T-cell lymphoma
  • Cutaneous T-cell lymphoproliferative disorder
  • Multispectral imaging analysis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Dermatology

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