Multispectral imaging fluorescence microscopy for living cells

Yasushi Hiraoka*, Takeshi Shimi, Tokuko Haraguchi

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

128 Scopus citations


Multispectral imaging technologies have been widely used in fields of astronomy and remote sensing. Interdisciplinary approaches developed in, for example, the National Aeronautics and Space Administration (NASA, USA), the Jet Propulsion Laboratory (JPL, USA), or the Communications Research Laboratory (CRL, Japan) have extended the application areas of these technologies from planetary systems to cellular systems. Here we overview multispectral imaging systems that have been devised for microscope applications. We introduce these systems with particular interest in live cell imaging. Finally we demonstrate examples of spectral imaging of living cells using commercially available systems with no need for user engineering.

Original languageEnglish (US)
Pages (from-to)367-374
Number of pages8
JournalCell Structure and Function
Issue number5
StatePublished - Oct 2002


  • Fluorescence resonance energy transfer
  • GFP
  • Linear unmixing
  • Spectroscopy

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology


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