@article{8311d58f5c1d488a930fba81460bb0fb,
title = "Multivalent Clustering of Adhesion Ligands in Nanofiber-Nanoparticle Composites",
abstract = "Because the positioning and clustering of biomolecules within the extracellular matrix dictates cell behaviors, the engineering of biomaterials incorporating bioactive epitopes with spatial organization tunable at the nanoscale is of primary importance. Here we used a highly modular composite approach combining peptide amphiphile (PA) nanofibers and silica nanoparticles, which are both easily functionalized with one or several bioactive signals. We show that the surface of silica nanoparticles allows the clustering of RGDS bioactive signals leading to improved adhesion and spreading of fibroblast cells on composite hydrogels at an epitope concentration much lower than in PA-only based matrices. Most importantly, by combining the two integrin-binding sequences RGDS and PHSRN on nanoparticle surfaces, we improved cell adhesion on the PA nanofiber/particle composite hydrogels, which is attributed to synergistic interactions known to be effective only for peptide intermolecular distance of ca. 5 nm. Such composites with soft and hard nanostructures offer a strategy for the design of advanced scaffolds to display multiple signals and control cell behavior.",
keywords = "Peptide amphiphile, clustering, hybrid biomaterials, nanobiocomposites, silica particles",
author = "Dounia Dems and Ronit Freeman and Riker, {Kyle D.} and Thibaud Coradin and Stupp, {Samuel I.} and Carole Aim{\'e}",
note = "Funding Information: Peptide synthesis was performed at the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. This facility has current support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS – 1542205). Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. SEM imaging was performed at the EPIC facility (NUANCE Center-Northwestern University), which has received support from the MRSEC program (NSF DMR-1121262) at the Materials Research Center; the Nanoscale Science and Engineering Center (NSF EEC-0647560) at the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN. We thank Mark Trosper McClendon for SEM observations. XPS work was performed with the assistance of Carrie Donley, PhD at the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN) which is funded by the National Science Foundation (NSF) (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Funding Information: Peptide synthesis was performed at the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. This facility has current support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS ? 1542205). Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. SEM imaging was performed at the EPIC facility (NUANCE Center-Northwestern University), which has received support from the MRSEC program (NSF DMR-1121262) at the Materials Research Center; the Nanoscale Science and Engineering Center (NSF EEC-0647560) at the International Institute for Nanotechnology (IIN); the Keck Foundation; and the State of Illinois, through the IIN. We thank Mark Trosper McClendon for SEM observations. XPS work was performed with the assistance of Carrie Donley, PhD at the Chapel Hill Analytical and Nanofabrication Laboratory (CHANL), a member of the North Carolina Research Triangle Nanotechnology Network (RTNN) which is funded by the National Science Foundation (NSF) (Grant ECCS-1542015) as part of the National Nanotechnology Coordinated Infrastructure (NNCI). Funding Information: This work was supported by an award from the Center for Regenerative Nanomedicine in the Simpson Querrey Institute at Northwestern University. Dounia Dems thanks the ED 397, Sorbonne University for her PhD grant, the Franco-American Commission for Educational Exchange for support through a Fulbright grant, and the student exchange program during her undergraduate studies organized by the Office of International Relations at Northwestern University between {\'E}cole Sup{\'e}rieure de Physique et de Chimie Industrielles in Paris and Northwestern University in Chicago, Illinois, USA, which initiated her connection with the laboratory of Samuel I. Stupp at the Simpson Querrey Institute. Publisher Copyright: {\textcopyright} 2020 Acta Materialia Inc.",
year = "2021",
month = jan,
day = "1",
doi = "10.1016/j.actbio.2020.11.009",
language = "English (US)",
volume = "119",
pages = "303--311",
journal = "Acta Biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
}