Murine interstitial nephritis. VIII. Characterization of Igh-V restriction determinants in the development of anti-idiotypic immunity using blocking antibodies

William H. Hines*, Richard A. Mann, Eric G. Neilson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have prepared antisera specific for Igh-V-linked determinants (αIgh-V) in order to study Igh-V restriction during the development of protective, T cell-mediated anti-idiotypic immunity in autoimmune interstitial nephritis. Suppressor T cells in this network use an antigen-binding (RE-Id+) factor (TsF1) secreted by first-order suppressor cells (Ts-1) to induce an anti-idiotypic (RE-αId+) soluble factor (TsF2) released by effectorphase, Ts-2 suppressor cells. Each of these soluble suppressor factors requires homology in the Igh-V region to complete its regulatory functions. Our αIgh-V antisera, adsorbed against network idiotypes, can interfere with the Igh-V restriction used in the induction of Ts-2 suppression by TsF1. The antisera bind both TsF1 and TsF2 in an allele-specific manner and are cytotoxic to induced Ts-2 cells, but not their precursors. These Igh-V determinants appear to behave like activation molecules. They lie outside of the ligand-binding site, do not map with the serologic binding of idiotype, and thus act as distinct associative-recognition elements in the maturation of anti-idiotypic immunity.

Original languageEnglish (US)
Pages (from-to)143-157
Number of pages15
JournalJournal of Autoimmunity
Volume1
Issue number2
DOIs
StatePublished - Apr 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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