Abstract
Sustained activation of muscarinic acetylcholine receptors on neuron-like NG108-15 hybrid cells reduces the number of [3H]quinuclidinyl benzilate binding sites per cell as much as 88%. The response occurs at concentrations of agonists commensurate with those needed to occupy receptors and inhibit adenylate cyclase. Decreases in steady-state receptor levels persist as long as activator remains present. Withdrawal of activator results in a slow increase in receptor levels that is blocked by cycloheximide. Activation shortens receptor half-life by a factor of nearly 4, indicating that regulation occurs at the level of receptor breakdown.
Original language | English (US) |
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Pages (from-to) | 506-512 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 90 |
Issue number | 2 |
DOIs | |
State | Published - Sep 27 1979 |
Funding
in part by grants to W.L.K. from of America and N.I.H. (1 ROl
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology