Muscarinic acetylcholine receptor regulation by accelerated rate of receptor loss

William L. Klein*, Neil Nathanson, Marshall Nirenberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

Sustained activation of muscarinic acetylcholine receptors on neuron-like NG108-15 hybrid cells reduces the number of [3H]quinuclidinyl benzilate binding sites per cell as much as 88%. The response occurs at concentrations of agonists commensurate with those needed to occupy receptors and inhibit adenylate cyclase. Decreases in steady-state receptor levels persist as long as activator remains present. Withdrawal of activator results in a slow increase in receptor levels that is blocked by cycloheximide. Activation shortens receptor half-life by a factor of nearly 4, indicating that regulation occurs at the level of receptor breakdown.

Original languageEnglish (US)
Pages (from-to)506-512
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume90
Issue number2
DOIs
StatePublished - Sep 27 1979

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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