Muscarinic receptors modulate N-, P-, and L-type Ca2+ currents in rat striatal neurons through parallel pathways

Angela R. Howe, D. James Surmeier*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

Muscarinic modulation of Calcium currents was studied in acutely isolated striatal neurons from the adult rat using the whole-cell configuration of the patch-clamp technique. Muscarinic agonists reduced calcium currents through two distinct signaling pathways. One pathway depended upon PTX-sensitive G- proteins and targeted N- and P-type currents. The other pathway depended upon PTX-insensitive G-proteins and was rendered inactive by high intracellular concentrations of BAPTA and targeted L-type currents. The modulation of N- and P-type currents was relieved by strong depolarizing prepulses, whereas the modulation of L-type currents was not. These findings support the proposition that parallel signaling pathways exist between muscarinic receptors and calcium channels.

Original languageEnglish (US)
Pages (from-to)458-469
Number of pages12
JournalJournal of Neuroscience
Volume15
Issue number1 I
DOIs
StatePublished - 1995

Keywords

  • acetylcholine
  • acute dissociation
  • dihydropyridine
  • muscarine
  • neuromodulation
  • patch clamp
  • ω- conotoxin
  • ω-agatoxin

ASJC Scopus subject areas

  • Neuroscience(all)

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