Abstract
Mutations in TRPV4 have been linked to three distinct axonal neuropathies. However, the pathogenic mechanism underlying these disorders remains unclear. Both gain and loss of calcium channel activity of the mutant TRPV4 have been suggested. Here, we show that the three previously reported TRPV4 mutant channels have a physiological localization and display an increased calcium channel activity, leading to increased cytotoxicity in three different cell types. Patch clamp experiments showed that cells expressing mutant TRPV4 have much larger whole-cell currents than those expressing the wild-type TRPV4 channel. Single channel recordings showed that the mutant channels have higher open probability, due to a modification of gating, and no change in single-channel conductance. These data support the hypothesis that a "gain of function" mechanism, possibly leading to increased intracellular calcium influx, underlies the pathogenesis of the TRPV4-linked axonal neuropathies, and may have immediate implications for designing rational therapies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 17281-17291 |
| Number of pages | 11 |
| Journal | Journal of Biological Chemistry |
| Volume | 286 |
| Issue number | 19 |
| DOIs | |
| State | Published - May 13 2011 |
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ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
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Mutant TRPV4-mediated toxicity is linked to increased constitutive function in axonal neuropathies. / Fecto, Faisal; Shi, Yong; Huda, Rafiq; Martina, Marco; Siddique, Teepu; Deng, Han-Xiang.
In: Journal of Biological Chemistry, Vol. 286, No. 19, 13.05.2011, p. 17281-17291.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mutant TRPV4-mediated toxicity is linked to increased constitutive function in axonal neuropathies
AU - Fecto, Faisal
AU - Shi, Yong
AU - Huda, Rafiq
AU - Martina, Marco
AU - Siddique, Teepu
AU - Deng, Han-Xiang
PY - 2011/5/13
Y1 - 2011/5/13
N2 - Mutations in TRPV4 have been linked to three distinct axonal neuropathies. However, the pathogenic mechanism underlying these disorders remains unclear. Both gain and loss of calcium channel activity of the mutant TRPV4 have been suggested. Here, we show that the three previously reported TRPV4 mutant channels have a physiological localization and display an increased calcium channel activity, leading to increased cytotoxicity in three different cell types. Patch clamp experiments showed that cells expressing mutant TRPV4 have much larger whole-cell currents than those expressing the wild-type TRPV4 channel. Single channel recordings showed that the mutant channels have higher open probability, due to a modification of gating, and no change in single-channel conductance. These data support the hypothesis that a "gain of function" mechanism, possibly leading to increased intracellular calcium influx, underlies the pathogenesis of the TRPV4-linked axonal neuropathies, and may have immediate implications for designing rational therapies.
AB - Mutations in TRPV4 have been linked to three distinct axonal neuropathies. However, the pathogenic mechanism underlying these disorders remains unclear. Both gain and loss of calcium channel activity of the mutant TRPV4 have been suggested. Here, we show that the three previously reported TRPV4 mutant channels have a physiological localization and display an increased calcium channel activity, leading to increased cytotoxicity in three different cell types. Patch clamp experiments showed that cells expressing mutant TRPV4 have much larger whole-cell currents than those expressing the wild-type TRPV4 channel. Single channel recordings showed that the mutant channels have higher open probability, due to a modification of gating, and no change in single-channel conductance. These data support the hypothesis that a "gain of function" mechanism, possibly leading to increased intracellular calcium influx, underlies the pathogenesis of the TRPV4-linked axonal neuropathies, and may have immediate implications for designing rational therapies.
UR - http://www.scopus.com/inward/record.url?scp=79955758732&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955758732&partnerID=8YFLogxK
U2 - 10.1074/jbc.M111.237685
DO - 10.1074/jbc.M111.237685
M3 - Article
C2 - 21454511
AN - SCOPUS:79955758732
VL - 286
SP - 17281
EP - 17291
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 19
ER -