Mutation of the priA gene of Neisseria gonorrhoeae affects DNA transformation and DNA repair

Kimberly A. Kline, H. Steven Seifert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


In Escherichia coli, PriA is central to the restart of chromosomal replication when replication fork progression is disrupted and is also involved in homologous recombination and DNA repair. To investigate the role of PriA in recombination and repair in Neisseria gonorrhoeae, we identified, cloned, and insertionally inactivated the gonococcal priA homologue. The priA mutant showed a growth deficiency and decreased DNA repair capability and was completely for deficient in DNA transformation compared to the isogenic parental strain. The priA mutant was also more sensitive to the oxidative damaging agents H 2O2 and cumene hydroperoxide compared to the parental strain. These phenotypes were complemented by supplying a functional copy of priA elsewhere in the chromosome. The N. gonorrhoeae priA mutant showed no alteration in the frequency of pilin antigenic variation. We conclude that PriA participates in DNA repair and DNA transformation processes but not in pilin antigenic variation.

Original languageEnglish (US)
Pages (from-to)5347-5355
Number of pages9
JournalJournal of bacteriology
Issue number15
StatePublished - Aug 2005

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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