Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines

Krishan Kumar, Qamar Rahman, Holger Schipper, Claudia Matschegewski, Dietmar Schiffmann, Thilo Papp*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Seven tumour suppressor genes (Chk1, Chk2, Apaf1, Rb1, p53, p16 INK4a and p14ARF) and two oncogenes (N-ras and BRAF) were screened in nine human malignant melanoma (HMM) cell lines for point mutations or small deletions/insertions by DGGE, TGGE and SCCP analysis. For the first time in human mesothelioma, Chk1 gene mutations were detected in two of the nine investigated HMM cell lines. P53 gene mutations were found in three cell lines and p16INK4a mutations in 5. Mutation of the Chk1 gene implies a novel disruption mechanism of the p53 pathway in HMM, without affecting p53 itself. According to our knowledge, this is the first mutation screening of Chk1, Chk2, Apaf1 and Rb1 in human malignant mesothelioma.

Original languageEnglish (US)
Pages (from-to)743-750
Number of pages8
JournalOncology reports
Volume14
Issue number3
DOIs
StatePublished - Sep 2005

Keywords

  • Chk1
  • DGGE
  • Mesothelioma
  • SSCP
  • TGGE
  • p53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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