Mutations in PRPF31 inhibit pre-mRNA splicing of rhodopsin gene and cause apoptosis of retinal cells

Liya Yuan, Mariko Kawada, Necat Havlioglu, Hao Tang, Jane Y. Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Mutations in human PRPF31 gene have been identified in patients with autosomal dominant retinitis pigmentosa (adRP). To begin to understand mechanisms by which defects in this general splicing factor cause retinal degeneration, we examined the relationship between PRPF31 and pre-mRNA splicing of photoreceptor-specific genes. We used a specific anti-PRPF31 antibody to immunoprecipitate splicing complexes from retinal cells and identified the transcript of rhodopsin gene (RHO) among RNA species associated with PRPF31-containing complexes. Mutant PRPF31 proteins significantly inhibited pre-mRNA splicing of intron 3 in RHO gene. In primary retinal cell cultures, expression of the mutant PRPF31 proteins reduced rhodopsin expression and caused apoptosis of rhodopsin-positive retinal cells. This primary retinal culture assay provides an in vitro model to study photoreceptor cell death caused by PRPF31 mutations. Our results demonstrate that mutations in PRPF31 gene affect RHO pre-mRNA splicing and reveal a link between PRPF31 and RHO, two major adRP genes.

Original languageEnglish (US)
Pages (from-to)748-757
Number of pages10
JournalJournal of Neuroscience
Volume25
Issue number3
DOIs
StatePublished - Jan 19 2005

Keywords

  • Apoptosis
  • Autosomal dominant retinitis pigmentosa (adRP)
  • PRPF31
  • Retinal cells
  • Rhodopsin
  • pre-mRNA splicing

ASJC Scopus subject areas

  • Neuroscience(all)

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