Myofibrillar creatine kinase activity is reduced in both short-term hibernation and myocardial stunning

K. Kozlowski*, A. Sherman, D. Evans, K. Harris, S. Hedibeli, F. Klocke

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

The role of damage to myofibrillar proteins in reversibly hypocontractile myocardium has been examined in chronically instrumented dogs undergoing sustained partial coronary occlusion. Findings in six animals 24 hours following a five-hour flow reduction of ∼75% were consistent with myocardial stunning, i.e., regional sonomicrometric function remained depressed (57 ± 7[SEM]% of control) while myocardial O2 consumption had returned to control levels . Findings in seven additional animals following a two-hour 50% flow reduction were consistent with myocardial hibernation, i.e., regional function and myocardial O2 consumption were reduced proportionately, averaging 80 ± 4% and 85 ± 4% of control. Creatine kinase (CK) activity in myofibrils isolated from areas of reduced flow was reduced systematically in both situations. In "stunned" animals subendocardial and full-thickness CK activities averaged 69 ± 9 and 83 ± 10% of levels in normally perfused myocardium (both p<0.05). Corresponding values in "hibernating" animals were 76 ± 11 and 79 " 11% (both p<0.05). CK activity was also reduced in animals undergoing two- or five-hour flow reductions without reperfusion. The CK reductions in both settings emphasize the complexity of responses to flow reduction, as well as limitations of the dichotomous characterization of post-ischemic contractile dysfunction as hibernating or stunned.

Original languageEnglish (US)
Pages (from-to)A1113
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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