Myofibrillar disruption in hypocontractile myocardium showing perfusion- contraction matches and mismatches

Andrew J. Sherman, Francis J. Klocke*, Robert S. Decker, Marlene L. Decker, Karen A. Kozlowski, Kathleen R. Harris, Sascha Hedjbeli, Yuri Yaroshenko, Sakie Nakamura, Michele A. Parker, Paul A. Checchia, Daniel B. Evans

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


Chronically instrumented dogs underwent 2- or 5-h regional reductions in coronary flow that were followed, respectively, by balanced reductions in myocardial contraction and O2 consumption ('hibernation') and persistently reduced contraction despite normal myocardial O2 consumption ('stunning'). Previously unidentified myofibrillar disruption developed during flow reduction in both experimental models and persisted throughout the duration of reperfusion (2-24 h). Aberrant perinuclear aggregates that resembled thick filaments and stained positively with a monoclonal myosin antibody were present in 34 ± 3.8% (SE) and 68 ± 5.9% of 'hibernating' and 'stunned' subendocardial myocytes in areas subjected to flow reduction and in 16 ± 2.5% and 44 ± 7.4% of subendocardial myocytes in remote areas of the same ventricles. Areas of myofibrillar disruption also showed glycogen accretion and unusual heterochromatin clumping adjacent to the inner nuclear envelope. The degrees of flow reduction employed were sufficient to reduce regional myofibrillar creatine kinase activity by 25-35%, but troponin I degradation was not evident. The observed changes may reflect an early, possibly reversible, phase of the myofibrillar loss characteristic of hypocontractile myocardium in patients undergoing revascularization.

Original languageEnglish (US)
Pages (from-to)H1320-H1334
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number4 47-4
StatePublished - Apr 2000


  • Apoptosis
  • Myocardial hibernation
  • Myocardial ischemia
  • Stress proteins
  • Stunning

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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