TY - JOUR
T1 - Myomir dysregulation and reactive oxygen species in aged human satellite cells
AU - Di Filippo, Ester Sara
AU - Mancinelli, Rosa
AU - Pietrangelo, Tiziana
AU - La Rovere, Rita Maria Laura
AU - Quattrocelli, Mattia
AU - Sampaolesi, Maurilio
AU - Fulle, Stefania
N1 - Funding Information:
We thank Dr. Vittore Verratti for his valuable skill in the needle-biopsies execution and Christofer Berrie for his manuscript generous editing. Sampaolesi is supported by Opening The Future Campaign (EJJ-OPTFUT-02010 ), CARE-MI FP7 ( #QPG-394601 ), AFM-Téléthon ( #18073 ), Programmafinanciering KUL PF/10/00300 , CARIPLO ( 2015-0634 ), FWO ( #G088715N , #G060612N , #G0A8813N ), GOA ( EJJ-C2161-GOA/11/012 ), IUAP-VII/07 ( EJJ-C4851-17/07-P ) and OT#09-053 ( EJJ-C0420-OT/09/053 ) grants. Mancinelli, Pietrangelo and Fulle are supported by University “G. d'Annunzio” of Chieti-Pescara local research grants and by MIUR research grants [ FIRB 2012 ( RBFR12BUMH_005 Mancinelli ), PRIN 2012 ( 2012N8YJC3_001 Pietrangelo ) and PRIN 2010-2011 ( 2010R8JK2X_007 Fulle )].
Publisher Copyright:
© 2016 The Authors.
PY - 2016/4/29
Y1 - 2016/4/29
N2 - Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.
AB - Satellite cells that reside on the myofibre surface are crucial for the muscle homeostasis and regeneration. Aging goes along with a less effective regeneration of skeletal muscle tissue mainly due to the decreased myogenic capability of satellite cells. This phenomenon impedes proper maintenance and contributes to the age-associated decline in muscle mass, known as sarcopenia. The myogenic potential impairment does not depend on a reduced myogenic cell number, but mainly on their difficulty to complete a differentiation program. The unbalanced production of reactive oxygen species in elderly people could be responsible for skeletal muscle impairments. microRNAs are conserved post-transcriptional regulators implicated in numerous biological processes including adult myogenesis. Here, we measure the ROS level and analyze myomiR (miR-1, miR-133b and miR-206) expression in human myogenic precursors obtained from Vastus lateralis of elderly and young subjects to provide the molecular signature responsible for the differentiation impairment of elderly activated satellite cells.
KW - Aging
KW - Human satellite cells
KW - ROS
KW - Sarcopenia
KW - myomiRNAs
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U2 - 10.1016/j.bbrc.2016.03.030
DO - 10.1016/j.bbrc.2016.03.030
M3 - Article
C2 - 26975470
AN - SCOPUS:84961778463
SN - 0006-291X
VL - 473
SP - 462
EP - 470
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -