Myosin and actin filament lengths in diaphragms from emphysematous hamsters

D. C. Poole*, R. L. Lieber, O. Mathieu-Costello

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

In vitro studies of the diaphragm from emphysematous animals have, in some instances, shown an alteration in its sarcomere length-tension relationship and a decreased maximal specific tension. To our knowledge, it has never been determined whether such functional changes may be indicative of ultrastructural adaptations, e.g., changes in filament lengths and thus cross-bridge number. To address this, we compared filament lengths in diaphragms from hamsters in which emphysema was induced by endotracheal instillation of elastase (E) 5 mo before the hamsters were killed with those from control hamsters (C; saline instillation). Diaphragms were then fixed by vascular perfusion with buffered glutaraldehyde in situ at airway pressures set to approximate the physiological range of lung volumes from residual volume (RV) to total lung capacity (TLC). Ultrathin sections (50-70 nm) were taken parallel to the muscle fiber axis and examined by electron microscopy (x33,000). Sarcomere and filament length measurements were calibrated using an actin periodicity of 39 nm and an M-band width of 86 nm to correct for dimensional changes during preparation. Emphysema increased the change in lung volume from -20 to +25 cmH2O airway pressure (from RV to TLC) by ~88%, and the displacement volume of excised lung at 0 cmH2O airway pressure was increased by ~138% on average. Neither myosin (C = 1.592 ± 0.027; E = 1.572 ± 0.035 μm; P = 0.72) nor actin (C = 1.210 ± 0.035; E = 1.221 ± 0.014 μm; P = 0.76) filament lengths were affected by emphysema. Thus, filament length changes do not underlie the diaphragm functional adaptations observed previously in emphysema.

Original languageEnglish (US)
Pages (from-to)1220-1225
Number of pages6
JournalJournal of applied physiology
Volume76
Issue number3
DOIs
StatePublished - 1994

Keywords

  • chronic obstructive pulmonary disease
  • length-tension relationship
  • muscle ultrastructure
  • sarcomere
  • specific tension

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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