Myosin-Va restrains the trafficking of Na+/K+-ATPase-containing vesicles in alveolar epithelial cells

Emilia Lecuona*, Alexander Minin, Humberto E. Trejo, Jiwang Chen, Alejandro P. Comellas, Haiying Sun, Doris Grillo, Oxana E. Nekrasova, Lynn C. Welch, Igal Szleifer, Vladimir I. Gelfand, Jacob I. Sznajder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Stimulation of Na+/K+-ATPase activity in alveolar epithelial cells by cAMP involves its recruitment from intracellular compartments to the plasma membrane. Here, we studied the role of the actin molecular motor myosin-V in this process. We provide evidence that, in alveolar epithelial cells, cAMP promotes Na+/K+-ATPase recruitment to the plasma membrane by increasing the average speed of Na+/K+-ATPase- containing vesicles moving to the cell periphery. We found that three isoforms of myosin-V are expressed in alveolar epithelial cells; however, only myosin-Va and Vc colocalized with the Na+/K+-ATPase in intracellular membrane fractions. Overexpression of dominant-negative myosin-Va or knockdown with specific shRNA increased the average speed and distance traveled by the Na+/K+-ATPase-containing vesicles, as well as the Na+/K+-ATPase activity and protein abundance at the plasma membrane to similar levels as those observed with cAMP stimulation. These data show that myosin-Va has a role in restraining Na+/K+-ATPase-containing vesicles within intracellular pools and that this restrain is released after stimulation by cAMP allowing the recruitment of the Na+/K+-ATPase to the plasma membrane and thus increased activity.

Original languageEnglish (US)
Pages (from-to)3915-3922
Number of pages8
JournalJournal of cell science
Volume122
Issue number21
DOIs
StatePublished - 2009

Keywords

  • Myosin-V
  • Na/K-ATPase
  • Traffic

ASJC Scopus subject areas

  • Cell Biology

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