Myosin VI is a processive motor with a large step size

Ronald S. Rock, Sarah E. Rice, Amber L. Wells, Thomas J. Purcell, James A. Spudich*, H. Lee Sweeney

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

308 Scopus citations

Abstract

Myosin VI is a molecular motor involved in intracellular vesicle and organelle transport. To carry out its cellular functions myosin VI moves toward the pointed end of actin, backward in relation to all other characterized myosins. Myosin V, a motor that moves toward the barbed end of actin, is processive, undergoing multiple catalytic cycles and mechanical advances before it releases from actin. Here we show that myosin VI is also processive by using single molecule motility and optical trapping experiments. Remarkably, myosin VI takes much larger steps than expected, based on a simple lever-arm mechanism, for a myosin with only one light chain in the lever-arm domain. Unlike other characterized myosins, myosin VI stepping is highly irregular with a broad distribution of step sizes.

Original languageEnglish (US)
Pages (from-to)13655-13659
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number24
DOIs
StatePublished - Nov 20 2001

ASJC Scopus subject areas

  • General

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