Myosin VI Walks "Wiggly" on Actin with Large and Variable Tilting

Yujie Sun, Harry W. Schroeder, John F. Beausang, Kazuaki Homma, Mitsuo Ikebe, Yale E. Goldman*

*Corresponding author for this work

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Myosin VI is an unconventional motor protein with unusual motility properties such as its direction of motion and path on actin and a large stride relative to its short lever arms. To understand these features, the rotational dynamics of the lever arm were studied by single-molecule polarized total internal reflection fluorescence (polTIRF) microscopy during processive motility of myosin VI along actin. The axial angle is distributed in two peaks, consistent with the hand-over-hand model. The changes in lever arm angles during discrete steps suggest that it exhibits large and variable tilting in the plane of actin and to the sides. These motions imply that, in addition to the previously suggested flexible tail domain, there is a compliant region between the motor domain and lever arm that allows myosin VI to accommodate the helical position of binding sites while taking variable step sizes along the actin filament.

Original languageEnglish (US)
Pages (from-to)954-964
Number of pages11
JournalMolecular cell
Volume28
Issue number6
DOIs
StatePublished - Dec 28 2007

Keywords

  • CELLBIO
  • PROTEINS

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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    Sun, Y., Schroeder, H. W., Beausang, J. F., Homma, K., Ikebe, M., & Goldman, Y. E. (2007). Myosin VI Walks "Wiggly" on Actin with Large and Variable Tilting. Molecular cell, 28(6), 954-964. https://doi.org/10.1016/j.molcel.2007.10.029