Nanodiscs as a modular platform for multimodal MR-optical imaging

Christiane E. Carney; Ivan L. Lenov; Catherine J. Baker; Keith W. Macrenaris; Amanda L. Eckermann; Stephen G. Sligar; Thomas J. Meade

doi:10.1021/acs.bioconjchem.5b00107

Nanodiscs as a modular platform for multimodal MR-optical imaging

Christiane E. Carney, Ivan L. Lenov, Catherine J. Baker, Keith W. Macrenaris, Amanda L. Eckermann, Stephen G. Sligar, Thomas J. Meade^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). Nanodiscs have been used to solubilize membrane proteins for structural and functional studies and deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs that solubilize lipophilic MR contrast agents for generation of multimodal nanoparticles for cellular imaging. We incorporate both multimeric and monomeric Gd(III)-based contrast agents into nanodiscs and show that particles containing the monomeric agent (ND2) label cells with high efficiency and generate significant image contrast at 7 T compared to nanodiscs containing the multimeric agent (ND1) and Prohance, a clinically approved contrast agent.

Original language	English (US)
Pages (from-to)	899-905
Number of pages	7
Journal	Bioconjugate Chemistry
Volume	26
Issue number	5
DOIs	https://doi.org/10.1021/acs.bioconjchem.5b00107
State	Published - May 20 2015

Funding

ASJC Scopus subject areas

Bioengineering
Biotechnology
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/acs.bioconjchem.5b00107

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title = "Nanodiscs as a modular platform for multimodal MR-optical imaging",

abstract = "Nanodiscs are monodisperse, self-assembled discoidal particles that consist of a lipid bilayer encircled by membrane scaffold proteins (MSP). Nanodiscs have been used to solubilize membrane proteins for structural and functional studies and deliver therapeutic phospholipids. Herein, we report on tetramethylrhodamine (TMR) tagged nanodiscs that solubilize lipophilic MR contrast agents for generation of multimodal nanoparticles for cellular imaging. We incorporate both multimeric and monomeric Gd(III)-based contrast agents into nanodiscs and show that particles containing the monomeric agent (ND2) label cells with high efficiency and generate significant image contrast at 7 T compared to nanodiscs containing the multimeric agent (ND1) and Prohance, a clinically approved contrast agent.",

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AU - Lenov, Ivan L.

AU - Baker, Catherine J.

AU - Macrenaris, Keith W.

AU - Eckermann, Amanda L.

AU - Sligar, Stephen G.

AU - Meade, Thomas J.

PY - 2015/5/20

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Nanodiscs as a modular platform for multimodal MR-optical imaging

Abstract

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ASJC Scopus subject areas

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