Nanoflares as probes for cancer diagnostics

Pratik S. Randeria, William E. Briley, Alyssa B. Chinen, Chenxia M. Guan, Sarah Hurst Petrosko, Chad A. Mirkin*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Patients whose cancer is detected early are much more likely to have a positive prognosis and outcome. Nanoflares hold promise as a practical diagnostic platform for the early detection of cancer markers in living cells. These probes are based on spherical nucleic acid (SNAs) and are typically composed of gold nanoparticle cores and densely packed and highly oriented oligonucleotide shells; these sequences are complementary to specific mRNA targets and are hybridized to fluorophore-labeled reporter strands. Nanoflares take advantage of the highly efficient fluorescence quenching properties of gold, the rapid cellular uptake of SNAs that occurs without the use of transfection agents, and the enzymatic stability of such constructs to report a highly sensitive and specific signal in the presence of intracellular target mRNA. In this chapter, we will focus on the synthesis, characterization, and diagnostic applications of nanoflares as they relate to cancer markers.

Original languageEnglish (US)
Pages (from-to)1-22
Number of pages22
JournalCancer treatment and research
Volume166
DOIs
StatePublished - Jan 1 2015

Keywords

  • Cancer diagnostics
  • Early stage cancer detection
  • Gold nanoparticles
  • Nanoflares
  • Spherical nucleic acids

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Nanoflares as probes for cancer diagnostics'. Together they form a unique fingerprint.

  • Cite this