Nanoflares for the Detection, Isolation, and Culture of Live Tumor Cells from Human Blood

Tiffany L. Halo; Kaylin M. McMahon; Nicholas L Angeloni; Yilin Xu; Wei Wang; Alyssa B. Chinen; Dmitry Malin; Elena Strekalova; Vincent L. Cryns; Chonghui Cheng; Chad A. Mirkin; C. Shad Thaxton

doi:10.1201/9781003056713-112

Nanoflares for the Detection, Isolation, and Culture of Live Tumor Cells from Human Blood

Tiffany L. Halo, Kaylin M. McMahon, Nicholas L Angeloni, Yilin Xu, Wei Wang, Alyssa B. Chinen, Dmitry Malin, Elena Strekalova, Vincent L. Cryns, Chonghui Cheng, Chad A. Mirkin, C. Shad Thaxton

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

1740Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. Nanoflares are nanoconstructs that enable live cell detection of intracellular mRNA. Nanoflares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, nanoflares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.

Original language	English (US)
Title of host publication	Spherical Nucleic Acids
Subtitle of host publication	Volume 4
Publisher	Jenny Stanford Publishing
Pages	1739-1759
Number of pages	21
Volume	4
ISBN (Electronic)	9781000092493
ISBN (Print)	9789814877244
DOIs	https://doi.org/10.1201/9781003056713-112
State	Published - Jan 1 2021

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Engineering
General Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1201/9781003056713-112

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Halo, T. L., McMahon, K. M., Angeloni, N. L., Xu, Y., Wang, W., Chinen, A. B., Malin, D., Strekalova, E., Cryns, V. L., Cheng, C., Mirkin, C. A., & Thaxton, C. S. (2021). Nanoflares for the Detection, Isolation, and Culture of Live Tumor Cells from Human Blood. In Spherical Nucleic Acids: Volume 4 (Vol. 4, pp. 1739-1759). Jenny Stanford Publishing. https://doi.org/10.1201/9781003056713-112

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abstract = "1740Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. Nanoflares are nanoconstructs that enable live cell detection of intracellular mRNA. Nanoflares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, nanoflares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.",

author = "Halo, {Tiffany L.} and McMahon, {Kaylin M.} and Angeloni, {Nicholas L} and Yilin Xu and Wei Wang and Chinen, {Alyssa B.} and Dmitry Malin and Elena Strekalova and Cryns, {Vincent L.} and Chonghui Cheng and Mirkin, {Chad A.} and Thaxton, {C. Shad}",

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doi = "10.1201/9781003056713-112",

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AU - Halo, Tiffany L.

AU - McMahon, Kaylin M.

AU - Angeloni, Nicholas L

AU - Xu, Yilin

AU - Wang, Wei

AU - Chinen, Alyssa B.

AU - Malin, Dmitry

AU - Strekalova, Elena

AU - Cryns, Vincent L.

AU - Cheng, Chonghui

AU - Mirkin, Chad A.

AU - Thaxton, C. Shad

PY - 2021/1/1

Y1 - 2021/1/1

N2 - 1740Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. Nanoflares are nanoconstructs that enable live cell detection of intracellular mRNA. Nanoflares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, nanoflares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.

AB - 1740Metastasis portends a poor prognosis for cancer patients. Primary tumor cells disseminate through the bloodstream before the appearance of detectable metastatic lesions. The analysis of cancer cells in blood-so-called circulating tumor cells (CTCs)-may provide unprecedented opportunities for metastatic risk assessment and investigation. Nanoflares are nanoconstructs that enable live cell detection of intracellular mRNA. Nanoflares, when coupled with flow cytometry, can be used to fluorescently detect genetic markers of CTCs in the context of whole blood. They allow one to detect as few as 100 live cancer cells per mL of blood and subsequently culture those cells. This technique can also be used to detect CTCs in a murine model of metastatic breast cancer. As such, nanoflares provide, to our knowledge, the first genetic-based approach for detecting, isolating, and characterizing live cancer cells from blood and may provide new opportunities for cancer diagnosis, prognosis, and personalized therapy.

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Nanoflares for the Detection, Isolation, and Culture of Live Tumor Cells from Human Blood

Abstract

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