Nanofountain-probe-based high-resolution patterning and single-cell injection of functionalized nanodiamonds

Owen Loh, Robert Lam, Mark Chen, Nicolaie Moldovan, Houjin Huang, Dean Ho*, Horacio Dante Espinosa

*Corresponding author for this work

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Nanodiamonds are rapidly emerging as promising carriers for nextgeneration therapeutics and drug delivery. However, developing future nanoscale devices and arrays that harness these nanoparticles will require unrealized spatial control. Furthermore, single-cell in vitro transfection methods lack an instrument that simultaneously offers the advantages of having nanoscale dimensions and control and continuous delivery via microfluidic components. To address this, two modes of controlled delivery of functionalized diamond nanoparticles are demonstrated using a broadly applicable nanofountain probe, a tool for direct-write nanopatterning with sub-100-nm resolution and direct in vitro single-cell injection. This study demonstrates the versatility of the nanofountain probe as a tool for highfidelity delivery of functionalized nanodiamonds and other agents in nanomanufacturing and single-cell biological studies. These initial demonstrations of controlled delivery open the door to future studies examining the nanofountain probe's potential in delivering specific doses of DNA, viruses, and other therapeutically relevant biomolecules.

Original languageEnglish (US)
Pages (from-to)1667-1674
Number of pages8
JournalSmall
Volume5
Issue number14
DOIs
StatePublished - Jul 17 2009

Keywords

  • Arrays
  • Drug delivery
  • Nanodiamonds
  • Nanoparticles
  • Nanopatterning

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

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