Nanofountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Postpulse Electropore Dynamics

Samba Shiva Prasad Nathamgari, Nibir Pathak, Vincent Lemaitre, Prithvijit Mukherjee, Joseph J. Muldoon, Chian Yu Peng, Tammy McGuire, Joshua N. Leonard, John A. Kessler, Horacio Dante Espinosa*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Introducing exogenous molecules into cells with high efficiency and dosage control is a crucial step in basic research as well as clinical applications. Here, the capability of the nanofountain probe electroporation (NFP-E) system to deliver proteins and plasmids in a variety of continuous and primary cell types with appropriate dosage control is reported. It is shown that the NFP-E can achieve fine control over the relative expression of two cotransfected plasmids. Finally, the dynamics of electropore closure after the pulsing ends with the NFP-E is investigated. Localized electroporation has recently been utilized to demonstrate the converse process of delivery (sampling), in which a small volume of the cytosol is retrieved during electroporation without causing cell lysis. Single-cell temporal sampling confers the benefit of monitoring the same cell over time and can provide valuable insights into the mechanisms underlying processes such as stem cell differentiation and disease progression. NFP-E parameters that maximize the membrane resealing time, which is essential for increasing the sampled volume and in meeting the challenge of monitoring low copy number biomarkers, are identified. Its application in CRISPR/Cas9 gene editing, stem cell reprogramming, and single-cell sampling studies is envisioned.

Original languageEnglish (US)
Article number2002616
JournalSmall
Volume16
Issue number43
DOIs
StatePublished - Oct 1 2020

Keywords

  • electroporation
  • nanopipettes
  • plasmid delivery
  • sampling
  • single cells

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Chemistry(all)
  • Materials Science(all)

Fingerprint Dive into the research topics of 'Nanofountain Probe Electroporation Enables Versatile Single-Cell Intracellular Delivery and Investigation of Postpulse Electropore Dynamics'. Together they form a unique fingerprint.

Cite this