Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting

Zongjie Wang; Hansen Wang; Sichun Lin; Sharif Ahmed; Stephane Angers; Edward H. Sargent; Shana O. Kelley

doi:10.1021/acs.nanolett.2c01018

Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting

Zongjie Wang, Hansen Wang, Sichun Lin, Sharif Ahmed, Stephane Angers, Edward H. Sargent, Shana O. Kelley^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Magnetic cell sorting is an enabling tool for the isolation of specific cellular subpopulations for downstream applications and requires the cells to be labeled by a sufficient number of magnetic nanoparticles to leverage magnetophoresis for efficient separation. This requirement makes it challenging to target weakly expressed biomarkers. Here, we developed a new approach that selectively and efficiently amplifies the magnetic labeling on cells through sequentially connected antibodies and nanoparticles delivered to the surface or interior of the cell. Using this approach, we achieved amplification up to 100-fold for surface and intracellular markers. We also demonstrated the utility of this assay for enabling high-performance magnetic cell sorting when it is applied to the analysis of rare tumor cells for cancer diagnosis and the purification of transfected CAR T cells for immunotherapy. The data presented demonstrate a useful tool for the stratification of rare cell subpopulations.

Original language	English (US)
Pages (from-to)	4774-4783
Number of pages	10
Journal	Nano letters
Volume	22
Issue number	12
DOIs	https://doi.org/10.1021/acs.nanolett.2c01018
State	Published - Jun 22 2022

Funding

We thank Dr. Azza Al-Mahrouki at the Centre for Pharmaceutical Oncology, Faculty of Pharmacy, University of Toronto, for access to the DLS, and Dr. Ali Darbandi at the SickKids Nanoscale Biomedical Imaging Facility, Hospital for Sick Children, for his help in TEM. This research was supported in part by the Canadian Institutes of Health Research (grant no. FDN-148415) and the Natural Sciences and Engineering Research Council of Canada (grant no. RGPIN-2016-06090). This research is part of the University of Toronto's Medicine by Design initiative, which receives funding from the Canada First Research Excellence Fund. Z.W. was supported by an Alexander Graham Bell Canada Graduate Scholarship and a Centre for Pharmaceutical Oncology Graduate Student Scholarship.

Keywords

cancer diagnosis
cell sorting
cell therapy
magnetic nanoparticles
microfluidics

ASJC Scopus subject areas

Condensed Matter Physics
Mechanical Engineering
Bioengineering
General Chemistry
General Materials Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acs.nanolett.2c01018

Cite this

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title = "Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting",

abstract = "Magnetic cell sorting is an enabling tool for the isolation of specific cellular subpopulations for downstream applications and requires the cells to be labeled by a sufficient number of magnetic nanoparticles to leverage magnetophoresis for efficient separation. This requirement makes it challenging to target weakly expressed biomarkers. Here, we developed a new approach that selectively and efficiently amplifies the magnetic labeling on cells through sequentially connected antibodies and nanoparticles delivered to the surface or interior of the cell. Using this approach, we achieved amplification up to 100-fold for surface and intracellular markers. We also demonstrated the utility of this assay for enabling high-performance magnetic cell sorting when it is applied to the analysis of rare tumor cells for cancer diagnosis and the purification of transfected CAR T cells for immunotherapy. The data presented demonstrate a useful tool for the stratification of rare cell subpopulations.",

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AU - Wang, Zongjie

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AU - Lin, Sichun

AU - Ahmed, Sharif

AU - Angers, Stephane

AU - Sargent, Edward H.

AU - Kelley, Shana O.

PY - 2022/6/22

Y1 - 2022/6/22

N2 - Magnetic cell sorting is an enabling tool for the isolation of specific cellular subpopulations for downstream applications and requires the cells to be labeled by a sufficient number of magnetic nanoparticles to leverage magnetophoresis for efficient separation. This requirement makes it challenging to target weakly expressed biomarkers. Here, we developed a new approach that selectively and efficiently amplifies the magnetic labeling on cells through sequentially connected antibodies and nanoparticles delivered to the surface or interior of the cell. Using this approach, we achieved amplification up to 100-fold for surface and intracellular markers. We also demonstrated the utility of this assay for enabling high-performance magnetic cell sorting when it is applied to the analysis of rare tumor cells for cancer diagnosis and the purification of transfected CAR T cells for immunotherapy. The data presented demonstrate a useful tool for the stratification of rare cell subpopulations.

AB - Magnetic cell sorting is an enabling tool for the isolation of specific cellular subpopulations for downstream applications and requires the cells to be labeled by a sufficient number of magnetic nanoparticles to leverage magnetophoresis for efficient separation. This requirement makes it challenging to target weakly expressed biomarkers. Here, we developed a new approach that selectively and efficiently amplifies the magnetic labeling on cells through sequentially connected antibodies and nanoparticles delivered to the surface or interior of the cell. Using this approach, we achieved amplification up to 100-fold for surface and intracellular markers. We also demonstrated the utility of this assay for enabling high-performance magnetic cell sorting when it is applied to the analysis of rare tumor cells for cancer diagnosis and the purification of transfected CAR T cells for immunotherapy. The data presented demonstrate a useful tool for the stratification of rare cell subpopulations.

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KW - cell therapy

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KW - microfluidics

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Nanoparticle Amplification Labeling for High-Performance Magnetic Cell Sorting

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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