Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations

Reza M. Mohamadi; Justin D. Besant; Adam Mepham; Brenda Green; Laili Mahmoudian; Thaddeus Gibbs; Ivaylo Ivanov; Anahita Malvea; Jessica Stojcic; Alison L. Allan; Lori E. Lowes; Edward H. Sargent; Robert K. Nam; Shana O. Kelley

doi:10.1002/anie.201409376

Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations

Reza M. Mohamadi, Justin D. Besant, Adam Mepham, Brenda Green, Laili Mahmoudian, Thaddeus Gibbs, Ivaylo Ivanov, Anahita Malvea, Jessica Stojcic, Alison L. Allan, Lori E. Lowes, Edward H. Sargent, Robert K. Nam, Shana O. Kelley

Chemistry

Research output: Contribution to journal › Article › peer-review

120 Scopus citations

Abstract

The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters bloodborne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial-to-mesenchymal transition (EMT).

Original language	English (US)
Pages (from-to)	139-143
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	54
Issue number	1
DOIs	https://doi.org/10.1002/anie.201409376
State	Published - Jan 2 2015

Keywords

Cancer cell analysis
Cell sorting
Magnetic nanoparticles
Microfluidics
Tumor cells

ASJC Scopus subject areas

Chemistry(all)
Catalysis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/anie.201409376

Cite this

Mohamadi, R. M., Besant, J. D., Mepham, A., Green, B., Mahmoudian, L., Gibbs, T., Ivanov, I., Malvea, A., Stojcic, J., Allan, A. L., Lowes, L. E., Sargent, E. H., Nam, R. K., & Kelley, S. O. (2015). Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations. Angewandte Chemie - International Edition, 54(1), 139-143. https://doi.org/10.1002/anie.201409376

@article{23e026c21ecd41d989a6852217a93a9c,

title = "Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations",

abstract = "The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters bloodborne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial-to-mesenchymal transition (EMT).",

keywords = "Cancer cell analysis, Cell sorting, Magnetic nanoparticles, Microfluidics, Tumor cells",

author = "Mohamadi, {Reza M.} and Besant, {Justin D.} and Adam Mepham and Brenda Green and Laili Mahmoudian and Thaddeus Gibbs and Ivaylo Ivanov and Anahita Malvea and Jessica Stojcic and Allan, {Alison L.} and Lowes, {Lori E.} and Sargent, {Edward H.} and Nam, {Robert K.} and Kelley, {Shana O.}",

note = "Publisher Copyright: {\textcopyright} 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2015",

month = jan,

day = "2",

doi = "10.1002/anie.201409376",

language = "English (US)",

volume = "54",

pages = "139--143",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "1",

}

Mohamadi, RM, Besant, JD, Mepham, A, Green, B, Mahmoudian, L, Gibbs, T, Ivanov, I, Malvea, A, Stojcic, J, Allan, AL, Lowes, LE, Sargent, EH, Nam, RK & Kelley, SO 2015, 'Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations', Angewandte Chemie - International Edition, vol. 54, no. 1, pp. 139-143. https://doi.org/10.1002/anie.201409376

TY - JOUR

T1 - Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations

AU - Mohamadi, Reza M.

AU - Besant, Justin D.

AU - Mepham, Adam

AU - Green, Brenda

AU - Mahmoudian, Laili

AU - Gibbs, Thaddeus

AU - Ivanov, Ivaylo

AU - Malvea, Anahita

AU - Stojcic, Jessica

AU - Allan, Alison L.

AU - Lowes, Lori E.

AU - Sargent, Edward H.

AU - Nam, Robert K.

AU - Kelley, Shana O.

PY - 2015/1/2

Y1 - 2015/1/2

N2 - The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters bloodborne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial-to-mesenchymal transition (EMT).

AB - The analysis of circulating tumor cells (CTCs) is an important capability that may lead to new approaches for cancer management. CTC capture devices developed to date isolate a bulk population of CTCs and do not differentiate subpopulations that may have varying phenotypes with different levels of clinical relevance. Here, we present a new device for CTC spatial sorting and profiling that sequesters bloodborne tumor cells with different phenotypes into discrete spatial bins. Validation data are presented showing that cancer cell lines with varying surface expression generate different binning profiles within the device. Working with patient blood samples, we obtain profiles that elucidate the heterogeneity of CTC populations present in cancer patients and also report on the status of CTCs within the epithelial-to-mesenchymal transition (EMT).

KW - Cancer cell analysis

KW - Cell sorting

KW - Magnetic nanoparticles

KW - Microfluidics

KW - Tumor cells

UR - http://www.scopus.com/inward/record.url?scp=84920123519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84920123519&partnerID=8YFLogxK

U2 - 10.1002/anie.201409376

DO - 10.1002/anie.201409376

M3 - Article

C2 - 25377874

AN - SCOPUS:84920123519

SN - 1433-7851

VL - 54

SP - 139

EP - 143

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 1

ER -

Nanoparticle-mediated binning and profiling of heterogeneous circulating tumor cell subpopulations

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this