Nanoparticle Transport from Mouse Vagina to Adjacent Lymph Nodes

Byron Ballou*, Susan K. Andreko, Elvira Osuna-Highley, Michael McRaven, Tina Catalone, Marcel P. Bruchez, Thomas J Hope, Mohamed E. Labib

*Corresponding author for this work

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

To test the feasibility of localized intravaginal therapy directed to neighboring lymph nodes, the transport of quantum dots across the vaginal wall was investigated. Quantum dots instilled into the mouse vagina were transported across the vaginal mucosa into draining lymph nodes, but not into distant nodes. Most of the particles were transported to the lumbar nodes; far fewer were transported to the inguinal nodes. A low level of transport was evident at 4 hr after intravaginal instillation, and transport peaked at about 36 hr after instillation. Transport was greatly enhanced by prior vaginal instillation of Nonoxynol-9. Hundreds of micrograms of nanoparticles/kg tissue (ppb) were found in the lumbar lymph nodes at 36 hr post-instillation. Our results imply that targeted transport of microbicides or immunogens from the vagina to local lymph organs is feasible. They also offer an in vivo model for assessing the toxicity of compounds intended for intravaginal use.

Original languageEnglish (US)
Article numbere51995
JournalPloS one
Volume7
Issue number12
DOIs
StatePublished - Dec 25 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Ballou, B., Andreko, S. K., Osuna-Highley, E., McRaven, M., Catalone, T., Bruchez, M. P., Hope, T. J., & Labib, M. E. (2012). Nanoparticle Transport from Mouse Vagina to Adjacent Lymph Nodes. PloS one, 7(12), [e51995]. https://doi.org/10.1371/journal.pone.0051995