Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

Jennifer C. Smith; Ki Bum Lee; Qian Wang; M. G. Finn; John E. Johnson; Milan Mrksich; Chad A. Mirkin

doi:10.1021/nl025956h

Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

Jennifer C. Smith, Ki Bum Lee, Qian Wang, M. G. Finn, John E. Johnson, Milan Mrksich^*, Chad A. Mirkin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

152 Scopus citations

Abstract

This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.

Original language	English (US)
Pages (from-to)	883-886
Number of pages	4
Journal	Nano letters
Volume	3
Issue number	7
DOIs	https://doi.org/10.1021/nl025956h
State	Published - Jul 1 2003

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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abstract = "This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.",

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T1 - Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

AU - Smith, Jennifer C.

AU - Lee, Ki Bum

AU - Wang, Qian

AU - Finn, M. G.

AU - Johnson, John E.

AU - Mrksich, Milan

AU - Mirkin, Chad A.

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AB - This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.

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Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

Abstract

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