Nanopatterning the chemospecific immobilization of cowpea mosaic virus capsid

Jennifer C. Smith, Ki Bum Lee, Qian Wang, M. G. Finn, John E. Johnson, Milan Mrksich*, Chad A. Mirkin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

150 Scopus citations


This paper presents a flexible approach for using Dip Pen Nanolithography (DPN) to nanopattern mixed monolayers for the selective immobilization of bioassemblies. DPN was used with a binary ink - consisting of a symmetric 11-mercaptoundecyl-penta(ethylene glycol) disulfide and a mixed disulfide substituted with one maleimide group - to pattern nanoscale features that present functional groups for the chemospecific immobilization of cysteine-labeled biomolecules. This strategy was applied to the chemospecific immobilization of cysteine mutant cowpea mosaic virus capsid particles (cys-VCPs). The combination of DPN for defining nanopatterns and surface chemistries for controlling the immobilization of ligands will be broadly useful in basic and applied biology.

Original languageEnglish (US)
Pages (from-to)883-886
Number of pages4
JournalNano letters
Issue number7
StatePublished - Jul 1 2003

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering


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