Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy

Adam Eshein*, Yue Li, Xiang Zhou, Graham Spicer, The Quyen Nguyen, Luay M. Almassalha, John E. Chandler, Scott Gladstein, Biqin Dong, Cheng Sun, Hao F Zhang, Vadim Backman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate a multimodal imaging system that combines partial-wave spectroscopic microscopy and spectroscopic super-resolution microscopy to study chromatin topology. This instrument allows nanoscale visualization of labeled and unlabeled chromatin with molecular and structural contrast.

Original languageEnglish (US)
Title of host publicationMicroscopy Histopathology and Analytics, MICROSCOPY 2018
PublisherOSA - The Optical Society
VolumePart F89-MICROSCOPY 2018
ISBN (Electronic)9781557528209
DOIs
StatePublished - Jan 1 2018
EventMicroscopy Histopathology and Analytics, MICROSCOPY 2018 - Hollywood, United States
Duration: Apr 3 2018Apr 6 2018

Other

OtherMicroscopy Histopathology and Analytics, MICROSCOPY 2018
CountryUnited States
CityHollywood
Period4/3/184/6/18

Fingerprint

Chromatin
Labels
Microscopic examination
Spectroscopy
Imaging techniques
Imaging systems
Visualization
Topology

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Mechanics of Materials

Cite this

Eshein, A., Li, Y., Zhou, X., Spicer, G., Nguyen, T. Q., Almassalha, L. M., ... Backman, V. (2018). Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy. In Microscopy Histopathology and Analytics, MICROSCOPY 2018 (Vol. Part F89-MICROSCOPY 2018). OSA - The Optical Society. https://doi.org/10.1364/MICROSCOPY.2018.MW2A.4
Eshein, Adam ; Li, Yue ; Zhou, Xiang ; Spicer, Graham ; Nguyen, The Quyen ; Almassalha, Luay M. ; Chandler, John E. ; Gladstein, Scott ; Dong, Biqin ; Sun, Cheng ; Zhang, Hao F ; Backman, Vadim. / Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy. Microscopy Histopathology and Analytics, MICROSCOPY 2018. Vol. Part F89-MICROSCOPY 2018 OSA - The Optical Society, 2018.
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abstract = "We demonstrate a multimodal imaging system that combines partial-wave spectroscopic microscopy and spectroscopic super-resolution microscopy to study chromatin topology. This instrument allows nanoscale visualization of labeled and unlabeled chromatin with molecular and structural contrast.",
author = "Adam Eshein and Yue Li and Xiang Zhou and Graham Spicer and Nguyen, {The Quyen} and Almassalha, {Luay M.} and Chandler, {John E.} and Scott Gladstein and Biqin Dong and Cheng Sun and Zhang, {Hao F} and Vadim Backman",
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Eshein, A, Li, Y, Zhou, X, Spicer, G, Nguyen, TQ, Almassalha, LM, Chandler, JE, Gladstein, S, Dong, B, Sun, C, Zhang, HF & Backman, V 2018, Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy. in Microscopy Histopathology and Analytics, MICROSCOPY 2018. vol. Part F89-MICROSCOPY 2018, OSA - The Optical Society, Microscopy Histopathology and Analytics, MICROSCOPY 2018, Hollywood, United States, 4/3/18. https://doi.org/10.1364/MICROSCOPY.2018.MW2A.4

Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy. / Eshein, Adam; Li, Yue; Zhou, Xiang; Spicer, Graham; Nguyen, The Quyen; Almassalha, Luay M.; Chandler, John E.; Gladstein, Scott; Dong, Biqin; Sun, Cheng; Zhang, Hao F; Backman, Vadim.

Microscopy Histopathology and Analytics, MICROSCOPY 2018. Vol. Part F89-MICROSCOPY 2018 OSA - The Optical Society, 2018.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy

AU - Eshein, Adam

AU - Li, Yue

AU - Zhou, Xiang

AU - Spicer, Graham

AU - Nguyen, The Quyen

AU - Almassalha, Luay M.

AU - Chandler, John E.

AU - Gladstein, Scott

AU - Dong, Biqin

AU - Sun, Cheng

AU - Zhang, Hao F

AU - Backman, Vadim

PY - 2018/1/1

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N2 - We demonstrate a multimodal imaging system that combines partial-wave spectroscopic microscopy and spectroscopic super-resolution microscopy to study chromatin topology. This instrument allows nanoscale visualization of labeled and unlabeled chromatin with molecular and structural contrast.

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VL - Part F89-MICROSCOPY 2018

BT - Microscopy Histopathology and Analytics, MICROSCOPY 2018

PB - OSA - The Optical Society

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Eshein A, Li Y, Zhou X, Spicer G, Nguyen TQ, Almassalha LM et al. Nanoscale imaging of chromatin with Labeled and Label- Free super-resolution microscopy and partial-wave spectroscopy. In Microscopy Histopathology and Analytics, MICROSCOPY 2018. Vol. Part F89-MICROSCOPY 2018. OSA - The Optical Society. 2018 https://doi.org/10.1364/MICROSCOPY.2018.MW2A.4