Nanoscale optical biosensors based on localized surface plasmon resonance spectroscopy

Amanda J. Haes*, Richard P. Van Duyne

*Corresponding author for this work

Research output: Contribution to journalConference article

44 Scopus citations

Abstract

The Ag nanoparticle based localized surface plasmon resonance (LSPR) nanosensor yields ultrasensitive biodetection with extremely simple, small, light, robust, and low-cost instrumentation. Using LSPR spectroscopy, the model system, biotinylated surface-confined Ag nanotriangles, was used to detect less than one picomolar up to micromolar concentrations of streptavidin. Additionally, the monitoring of anti-biotin binding to biotinylated Ag nanotriangles exhibited that the system could be used as a solution immunoassay. The system was rigorously tested for nonspecific binding interactions and was found to display virtually no adverse results. These results represent important new steps in the development of the LSPR nanobiosensor for applications in medical diagnostics, biomedical research, and environmental science.

Original languageEnglish (US)
Pages (from-to)47-58
Number of pages12
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5221
DOIs
StatePublished - Dec 1 2003
EventPlasmonics: Metallic Nanostuctures and Their Optical Properties - San Diego, CA, United States
Duration: Aug 3 2003Aug 5 2003

Keywords

  • Anti-biotin
  • Biosensors
  • Nanoparticles
  • Nanosphere lithography
  • Plasmon resonance
  • Streptavidin

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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