Abstract
The Ag nanoparticle based localized surface plasmon resonance (LSPR) nanosensor yields ultrasensitive biodetection with extremely simple, small, light, robust, and low-cost instrumentation. Using LSPR spectroscopy, the model system, biotinylated surface-confined Ag nanotriangles, was used to detect less than one picomolar up to micromolar concentrations of streptavidin. Additionally, the monitoring of anti-biotin binding to biotinylated Ag nanotriangles exhibited that the system could be used as a solution immunoassay. The system was rigorously tested for nonspecific binding interactions and was found to display virtually no adverse results. These results represent important new steps in the development of the LSPR nanobiosensor for applications in medical diagnostics, biomedical research, and environmental science.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 47-58 |
| Number of pages | 12 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 5221 |
| DOIs | |
| State | Published - Dec 1 2003 |
| Event | Plasmonics: Metallic Nanostuctures and Their Optical Properties - San Diego, CA, United States Duration: Aug 3 2003 → Aug 5 2003 |
Keywords
- Anti-biotin
- Biosensors
- Nanoparticles
- Nanosphere lithography
- Plasmon resonance
- Streptavidin
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering