Nanoscale refractive index fluctuations detected via sparse spectral microscopy

John E. Chandler, Lusik Cherkezyan, Hariharan Subramanian, Vadim Backman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Partial Wave Spectroscopic (PWS) Microscopy has proven effective at detecting nanoscale hallmarks of carcinogenesis in histologically normal-appearing cells. The current method of data analysis requires acquisition of a three-dimensional data cube, consisting of multiple images taken at different illumination wavelengths, limiting the technique to data acquisition on ~30 individual cells per slide. To enable high throughput data acquisition and whole-slide imaging, new analysis procedures were developed that require fewer wavelengths in the same 500-700nm range for spectral analysis. The nanoscale sensitivity of the new analysis techniques was validated (i) theoretically, using finite-difference time-domain solutions of Maxwell’s equations, as well as (ii) experimentally, by measuring nanostructural alterations associated with carcinogenesis in biological cells.

Original languageEnglish (US)
Article number252091
Pages (from-to)883-893
Number of pages11
JournalBiomedical Optics Express
Volume7
Issue number3
DOIs
StatePublished - Feb 19 2016

Keywords

  • Clinical applications
  • Imaging systems
  • Medical optics and biotechnology
  • Multispectral and hyperspectral imaging
  • Optical diagnostics for medicine
  • Spectroscopy
  • Tissue diagnostics

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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