TY - JOUR
T1 - Nanoscale refractive index fluctuations detected via sparse spectral microscopy
AU - Chandler, John E.
AU - Cherkezyan, Lusik
AU - Subramanian, Hariharan
AU - Backman, Vadim
N1 - Funding Information:
The authors would like to thank Justin Derbas and Charles Maneval for assistance with cell imaging. The results presented here are based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant DGE-0824162, the National Institutes of Health under Grants: R01CA200064, R01CA155284, R01CA165309, and R01EB016983, the Nation Science Foundation Small Business Innovation Research program under Grant IIP-1214989, and the National Institutes of Health Small Business Innovation Research program under Grant 1R44CA168185. Drs. Subramanian and Backman are cofounders and/or shareholders in Nanocytomics LLC. All aspects of this study were done under the supervision of the Conflict of Interest Committee at Northwestern University
Publisher Copyright:
© 2016 Optical Society of America.
PY - 2016/2/19
Y1 - 2016/2/19
N2 - Partial Wave Spectroscopic (PWS) Microscopy has proven effective at detecting nanoscale hallmarks of carcinogenesis in histologically normal-appearing cells. The current method of data analysis requires acquisition of a three-dimensional data cube, consisting of multiple images taken at different illumination wavelengths, limiting the technique to data acquisition on ~30 individual cells per slide. To enable high throughput data acquisition and whole-slide imaging, new analysis procedures were developed that require fewer wavelengths in the same 500-700nm range for spectral analysis. The nanoscale sensitivity of the new analysis techniques was validated (i) theoretically, using finite-difference time-domain solutions of Maxwell’s equations, as well as (ii) experimentally, by measuring nanostructural alterations associated with carcinogenesis in biological cells.
AB - Partial Wave Spectroscopic (PWS) Microscopy has proven effective at detecting nanoscale hallmarks of carcinogenesis in histologically normal-appearing cells. The current method of data analysis requires acquisition of a three-dimensional data cube, consisting of multiple images taken at different illumination wavelengths, limiting the technique to data acquisition on ~30 individual cells per slide. To enable high throughput data acquisition and whole-slide imaging, new analysis procedures were developed that require fewer wavelengths in the same 500-700nm range for spectral analysis. The nanoscale sensitivity of the new analysis techniques was validated (i) theoretically, using finite-difference time-domain solutions of Maxwell’s equations, as well as (ii) experimentally, by measuring nanostructural alterations associated with carcinogenesis in biological cells.
KW - Clinical applications
KW - Imaging systems
KW - Medical optics and biotechnology
KW - Multispectral and hyperspectral imaging
KW - Optical diagnostics for medicine
KW - Spectroscopy
KW - Tissue diagnostics
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UR - http://www.scopus.com/inward/citedby.url?scp=84961661968&partnerID=8YFLogxK
U2 - 10.1364/BOE.7.000883
DO - 10.1364/BOE.7.000883
M3 - Article
C2 - 27231596
AN - SCOPUS:84961661968
SN - 2156-7085
VL - 7
SP - 883
EP - 893
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 3
M1 - 252091
ER -