Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma

Andrea Baccarelli; Franca Rusconi; Valentina Bollati; Dolores Catelan; Gabriele Accetta; Lifang Hou; Fabio Barbone; Pier Alberto Bertazzi; Annibale Biggeri

doi:10.2217/epi.11.106

Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma

Andrea Baccarelli^*, Franca Rusconi, Valentina Bollati, Dolores Catelan, Gabriele Accetta, Lifang Hou, Fabio Barbone, Pier Alberto Bertazzi, Annibale Biggeri

^*Corresponding author for this work

Preventive Medicine

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

Original language	English (US)
Pages (from-to)	91-100
Number of pages	10
Journal	Epigenomics
Volume	4
Issue number	1
DOIs	https://doi.org/10.2217/epi.11.106
State	Published - Feb 2012

Keywords

DNA methylation
airway obstruction
asthma
children
epigenetics
inflammation

ASJC Scopus subject areas

Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/epi.11.106

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title = "Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma",

abstract = "Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.",

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author = "Andrea Baccarelli and Franca Rusconi and Valentina Bollati and Dolores Catelan and Gabriele Accetta and Lifang Hou and Fabio Barbone and Bertazzi, {Pier Alberto} and Annibale Biggeri",

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AU - Baccarelli, Andrea

AU - Rusconi, Franca

AU - Bollati, Valentina

AU - Catelan, Dolores

AU - Accetta, Gabriele

AU - Hou, Lifang

AU - Barbone, Fabio

AU - Bertazzi, Pier Alberto

AU - Biggeri, Annibale

PY - 2012/2

Y1 - 2012/2

N2 - Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

AB - Aims: DNA methylation is increasingly proposed as a mechanism for underlying asthma-related inflammation. However, epigenetic studies are constrained by uncertainties on whether samples that can be easily collected in human individuals can provide informative results. Methods: Two nasal cell DNA samples were collected on different days by nasal brushings from 35 asthmatic children aged between 8 and 11 years old. We correlated DNA methylation of IL-6, iNOS, Alu and LINE-1 with fractional exhaled nitric oxide, forced expiratory volume in 1 s and wheezing. Results: Fractional exhaled nitric oxide increased in association with lower promoter methylation of both IL-6 (+29.0%; p = 0.004) and iNOS (+41.0%; p = 0.002). Lower IL-6 methylation was nonsignificantly associated with wheezing during the week of the study (odds ratio = 2.3; p = 0.063). Conclusion: Our findings support the use of nasal cell DNA for human epigenetic studies of asthma.

KW - DNA methylation

KW - airway obstruction

KW - asthma

KW - children

KW - epigenetics

KW - inflammation

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Nasal cell DNA methylation, inflammation, lung function and wheezing in children with asthma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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