Naturally acquired immunity against immature Plasmodium falciparum gametocytes

Kathleen W. Dantzler, Siyuan Ma, Priscilla Ngotho, Will J.R. Stone, Dingyin Tao, Sanna Rijpma, Mariana De Niz, Sandra K.Nilsson Bark, Matthijs M. Jore, Tonke K. Raaijmakers, Angela M. Early, Ceereena Ubaida-Mohien, Leandro Lemgruber, Joseph J. Campo, Andy A. Teng, Timothy Q. Le, Cassidy L. Walker, Patricia Hermand, Philippe Deterre, D. Huw DaviesPhil Felgner, Isabelle Morlais, Dyann F. Wirth, Daniel E. Neafsey, Rhoel R. Dinglasan, Miriam Laufer, Curtis Huttenhower, Karl Seydel, Terrie Taylor, Teun Bousema, Matthias Marti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations


The recent decline in global malaria burden has stimulated efforts toward Plasmodium falciparum elimination. Understanding the biology of malaria transmission stages may provide opportunities to reduce or prevent onward transmission to mosquitoes. Immature P. falciparum transmission stages, termed stages I to IV gametocytes, sequester in human bone marrow before release into the circulation as mature stage V gametocytes. This process likely involves interactions between host receptors and potentially immunogenic adhesins on the infected red blood cell (iRBC) surface. Here, we developed a flow cytometry assay to examine immune recognition of live gametocytes of different developmental stages by naturally exposed Malawians. We identified strong antibody recognition of the earliest immature gametocyte-iRBCs (giRBCs) but not mature stage V giRBCs. Candidate surface antigens (n = 30), most of them shared between asexual-and gametocyte-iRBCs, were identified by mass spectrometry and mouse immunizations, as well as correlations between responses by protein microarray and flow cytometry. Naturally acquired responses to a subset of candidate antigens were associated with reduced asexual and gametocyte density, and plasma samples from malaria-infected individuals were able to induce immune clearance of giRBCs in vitro. Infected RBC surface expression of select candidate antigens was validated using specific antibodies, and genetic analysis revealed a subset with minimal variation across strains. Our data demonstrate that humoral immune responses to immature giRBCs and shared iRBC antigens are naturally acquired after malaria exposure. These humoral immune responses may have consequences for malaria transmission potential by clearing developing gametocytes, which could be leveraged for malaria intervention.

Original languageEnglish (US)
Article numbereaav3963
JournalScience translational medicine
Issue number495
StatePublished - Jun 5 2019

ASJC Scopus subject areas

  • Medicine(all)


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