Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry

Sehyun Kim, Norann A. Zaghloul, Ekaterina Bubenshchikova, Edwin C. Oh, Susannah Rankin, Elias Nicholas Katsanis, Tomoko Obara, Leonidas Tsiokas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

164 Scopus citations

Abstract

The primary cilium is an antenna-like organelle that is dynamically regulated during the cell cycle. Ciliogenesis is initiated as cells enter quiescence, whereas resorption of the cilium precedes mitosis. The mechanisms coordinating ciliogenesis with the cell cycle are unknown. Here we identify the centrosomal protein Nde1 (nuclear distribution gene E homologue 1) as a negative regulator of ciliary length. Nde1 is expressed at high levels in mitosis, low levels in quiescence and localizes at the mother centriole, which nucleates the primary cilium. Cells depleted of Nde1 have longer cilia and a delay in cell cycle re-entry that correlates with ciliary length. Knockdown of Nde1 in zebrafish embryos results in increased ciliary length, suppression of cell division, reduction of the number of cells forming the Kupffer's vesicle and left-ht patterning defects. These data suggest that Nde1 is an integral component of a network coordinating ciliary length with cell cycle progression and have implications for understanding the transition from a quiescent to a proliferative state.

Original languageEnglish (US)
Pages (from-to)351-362
Number of pages12
JournalNature Cell Biology
Volume13
Issue number4
DOIs
StatePublished - Apr 2011

ASJC Scopus subject areas

  • Cell Biology

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