Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Elias Gounaris; Yasushige Ishihara; Manisha Shrivastrav; David Bentrem; Terrence A. Barrett

doi:10.1007/978-1-4939-3603-8_13

Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Elias Gounaris, Yasushige Ishihara, Manisha Shrivastrav, David Bentrem, Terrence A. Barrett

Surgery, Surgical Oncology Division

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

Original language	English (US)
Pages (from-to)	137-147
Number of pages	11
Journal	Methods in Molecular Biology
Volume	1422
DOIs	https://doi.org/10.1007/978-1-4939-3603-8_13
State	Published - 2016

Keywords

Cathepsin activity
Colon cancer
Endoscopy
Mouse colon dysplasia
Mucosal inflammation
Near-infrared probes

ASJC Scopus subject areas

Molecular Biology
Genetics

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-4939-3603-8_13

Cite this

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title = "Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon",

abstract = "Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.",

keywords = "Cathepsin activity, Colon cancer, Endoscopy, Mouse colon dysplasia, Mucosal inflammation, Near-infrared probes",

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AU - Gounaris, Elias

AU - Ishihara, Yasushige

AU - Shrivastrav, Manisha

AU - Bentrem, David

AU - Barrett, Terrence A.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2016.

PY - 2016

Y1 - 2016

N2 - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

AB - Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

KW - Cathepsin activity

KW - Colon cancer

KW - Endoscopy

KW - Mouse colon dysplasia

KW - Mucosal inflammation

KW - Near-infrared probes

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Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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