Near-infrared fluorescence endoscopy to detect dysplastic lesions in the mouse colon

Elias Gounaris, Yasushige Ishihara, Manisha Shrivastrav, David Bentrem, Terrence A. Barrett

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Near-infrared fluorescence (NIRF) endoscopy has a great potential for efficient early detection of dysplastic lesions in the colon. For preclinical studies, we developed a small animal NIRF endoscope and successfully used this device to identify dysplastic lesions in a murine model of chronic colitis. In this chapter, we present a step-by-step protocol for using NIRF endoscopy to examine the location, the size, and the borders of the dysplastic lesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitisassociated colon cancer.

Original languageEnglish (US)
Pages (from-to)137-147
Number of pages11
JournalMethods in Molecular Biology
Volume1422
DOIs
StatePublished - Jan 1 2016

Keywords

  • Cathepsin activity
  • Colon cancer
  • Endoscopy
  • Mouse colon dysplasia
  • Mucosal inflammation
  • Near-infrared probes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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