Necrotic cells trigger a sterile inflammatory response through the Nlrp3 inflammasome

Shankar S. Iyer, Wilco P. Pulskens, Jeffrey J. Sadler, Loes M. Butter, Gwendoline J. Teske, Tyler K. Ulland, Stephanie C. Eisenbarth, Sandrine Florquin, Richard A. Flavell, Jaklien C. Leemans, Fayyaz S. Sutterwala

Research output: Contribution to journalArticlepeer-review

573 Scopus citations

Abstract

Dying cells are capable of activating the innate immune system and inducing a sterile inflammatory response. Here, we show that necrotic cells are sensed by the Nlrp3 inflammasome resulting in the subsequent release of the proinflammatory cytokine IL-1β. Necrotic cells produced by pressure disruption, hypoxic injury, or complement-mediated damage were capable of activating the Nlrp3 inflammasome. Nlrp3 inflammasome activation was triggered in part through ATP produced by mitochondria released from damaged cells. Neutrophilic influx into the peritoneum in response to necrotic cells in vivo was also markedly diminished in the absence of Nlrp3. Nlrp3-deficiency moreover protected animals against mortality, renal dysfunction, and neutrophil influx in an in vivo renal ischemic acute tubular necrosis model. These findings suggest that the inhibition of Nlrp3 inflammasome activity can diminish the acute inflammation and damage associated with tissue injury.

Original languageEnglish (US)
Pages (from-to)20388-20393
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number48
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Keywords

  • Caspase-1
  • Interleukin-1β
  • Necrosis

ASJC Scopus subject areas

  • General

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