Neomycin Inhibition of the Hammerhead Ribozyme Involves Ionic Interactions

B. Clouet-D'Orval, T. K. Stage, O. C. Uhlenbeck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

106 Scopus citations

Abstract

To investigate the properties of the neomycin-hammerhead interaction, inhibition of hammerhead activity was measured as a function of magnesium concentration and pH. The data are consistent with a simple competition between magnesium and neomycin with about five magnesium ions required to displace neomycin from the hammerhead. The pH dependence of the inhibition of hammerhead cleavage by neomycin and two related aminoglycosides was also determined. The data indicate that at least three of the five positively charged ammonium ions present on neomycin are critical for inhibiting hammerhead function. Taken together, these results suggest that the neomycin-hammerhead interaction is mostly ionic in character.

Original languageEnglish (US)
Pages (from-to)11186-11190
Number of pages5
JournalBiochemistry
Volume34
Issue number35
DOIs
StatePublished - Sep 1995

ASJC Scopus subject areas

  • Biochemistry

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